Journal article
The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
PeerJ, Vol.9, Art. e10280
2021
Abstract
It is now appreciated that long non-coding RNAs (lncRNAs) are important players in orchestrating cancer progression. In this study we characterized GHSROS, a human lncRNA gene on the opposite DNA strand (antisense) to the ghrelin receptor gene, in prostate cancer. The lncRNA was upregulated by prostate tumors from different clinical datasets. Transcriptome data revealed that GHSROS alters the expression of cancer-associated genes. Functional analyses in vitro showed that GHSROS mediates tumor growth, migration and survival, and resistance to the cytotoxic drug docetaxel. Increased cellular proliferation of GHSROS-overexpressing PC3, DU145, and LNCaP prostate cancer cell lines in vitro was recapitulated in a subcutaneous xenograft model. Conversely, in vitro antisense oligonucleotide inhibition of the lncRNA reciprocally regulated cell growth and migration, and gene expression. Notably, GHSROS modulates the expression of PPP2R2C, the loss of which may drive androgen receptor pathway-independent prostate tumor progression in a subset of prostate cancers. Collectively, our findings suggest that GHSROS can reprogram prostate cancer cells toward a more aggressive phenotype and that this lncRNA may represent a potential therapeutic target.
Details
- Title
- The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
- Authors/Creators
- P.B. Thomas (Author/Creator) - Translational Research InstituteP. Jeffery (Author/Creator) - Translational Research InstituteM.D. Gahete (Author/Creator) - Instituto Maimónides de Investigación Biomédica de CórdobaE. Whiteside (Author/Creator) - University of Southern QueenslandC. Walpole (Author/Creator) - Translational Research InstituteM. Maugham (Author/Creator) - Translational Research InstituteL. Jovanovic (Author/Creator) - Queensland University of TechnologyJ. Gunter (Author/Creator) - Queensland University of TechnologyE. Williams (Author/Creator) - Queensland University of TechnologyC. Nelson (Author/Creator) - Queensland University of TechnologyA. Herington (Author/Creator) - Translational Research InstituteR.M. Luque (Author/Creator) - Instituto Maimónides de Investigación Biomédica de CórdobaR. Veedu (Author/Creator) - Murdoch UniversityL.K. Chopin (Author/Creator) - Translational Research InstituteI. Seim (Author/Creator) - Translational Research Institute
- Publication Details
- PeerJ, Vol.9, Art. e10280
- Publisher
- PeerJ
- Identifiers
- 991005540156707891
- Copyright
- 2021 Thomas et al.
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.147 Prostate Cancer
- 1.147.859 Androgen Receptor
- Web Of Science research areas
- Oncology
- ESI research areas
- Multidisciplinary