The metabolic environment of the developing embryo: A multidisciplinary approach on oilseed rapeseed

H. Rolletschek; S. Mayer; B. Boughton; S. Wagner; S. Ortleb; C. Kiel; U. Roessner; L. Borisjuk

doi:10.1016/j.jplph.2021.153505

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The metabolic environment of the developing embryo: A multidisciplinary approach on oilseed rapeseed

Journal article

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The metabolic environment of the developing embryo: A multidisciplinary approach on oilseed rapeseed

H. Rolletschek, S. Mayer, B. Boughton, S. Wagner, S. Ortleb, C. Kiel, U. Roessner and L. Borisjuk

Journal of Plant Physiology, Vol.265, Art. 153505

2021

DOI: https://doi.org/10.1016/j.jplph.2021.153505

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Abstract

Brassicaceae seeds consist of three genetically distinct structures: the embryo, endosperm and seed coat, all of which are involved in assimilate allocation during seed development. The complexity of their metabolic interrelations remains unresolved to date. In the present study, we apply state-of-the-art imaging and analytical approaches to assess the metabolic environment of the Brassica napus embryo. Nuclear magnetic resonance imaging (MRI) provided volumetric data on the living embryo and endosperm, revealing how the endosperm envelops the embryo, determining endosperm's priority in assimilate uptake from the seed coat during early development. MRI analysis showed higher levels of sugars in the peripheral endosperm facing the seed coat, but a lower sugar content within the central vacuole and the region surrounding the embryo. Feeding intact siliques with 13C-labeled sucrose allowed tracing of the post-phloem route of sucrose transfer within the seed at the heart stage of embryogenesis, by means of mass spectrometry imaging. Quantification of over 70 organic and inorganic compounds in the endosperm revealed shifts in their abundance over different stages of development, while sugars and potassium were the main determinants of osmolality throughout these stages. Our multidisciplinary approach allows access to the hidden aspects of endosperm metabolism, a task which remains unattainable for the small-seeded model plant Arabidopsis thaliana.

Details

Title: The metabolic environment of the developing embryo: A multidisciplinary approach on oilseed rapeseed
Authors/Creators: H. Rolletschek (Author/Creator) - Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
S. Mayer (Author/Creator) - Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
B. Boughton (Author/Creator) - Murdoch University
S. Wagner (Author/Creator) - Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
S. Ortleb (Author/Creator) - Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
C. Kiel (Author/Creator)
U. Roessner (Author/Creator) - The University of Melbourne
L. Borisjuk (Author/Creator) - Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
Publication Details: Journal of Plant Physiology, Vol.265, Art. 153505
Publisher: Elsevier GmbH
Identifiers: 991005541195007891
Copyright: © 2021 The Author(s).
Murdoch Affiliation: Australian National Phenome Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.159 Arabidopsis
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science