Journal article
The metabolic fate and effects of 2-Bromophenol in male Sprague-Dawley rats
Xenobiotica, Vol.49(11), pp.1352-1359
2019
PMID: 30557119
Abstract
1. The metabolic fate and urinary excretion of 2-bromophenol, a phenolic metabolite of bromobenzene, was investigated in male Sprague–Dawley rats following single intraperitoneal doses at either 0, 100, or 200 mg/kg.
2. Urine was collected for seven days and samples analysed using 1 H NMR spectroscopy, inductively coupled plasma (ICP)MS, and UPLC-MS.
3. 1 H NMR spectroscopy of the urine samples showed that, at these doses, 2-bromophenol had little effect on endogenous metabolite profiles, supporting histopathology and clinical chemistry data, which showed no changes associated with the administration of 2-bromophenol in this study.
4. The use of ICP-MS provided a means for the selective detection and quantification of bromine-containing species and showed that between 15 and 30% of the dose was excreted via the urine over 7 days of the study for both the 100 and 200 mg doses, respectively.
5. The bulk of the excretion of Br-containing material had occurred by 8 h post administration. UPLC-MS of urine revealed a number of metabolites of 2-bromophenol, with 2-bromophenol glucuronide and 2-bromophenol sulphate identified as the major species. A number of minor hydroxylated metabolites were also detected as their glucuronide, sulphate, or O-methyl conjugates. There was no evidence for the production of reactive metabolites.
Details
- Title
- The metabolic fate and effects of 2-Bromophenol in male Sprague-Dawley rats
- Authors/Creators
- Kyrillos N. Adesina-Georgiadis - Imperial College LondonNicola Gray - University of ReadingRobert S. Plumb - Imperial College LondonDavid F. Thompson - Keele UniversityElaine Holmes - Imperial College LondonJeremy K. Nicholson - Imperial College LondonIan D. Wilson - Imperial College London
- Publication Details
- Xenobiotica, Vol.49(11), pp.1352-1359
- Publisher
- Taylor & Francis
- Number of pages
- 8
- Identifiers
- 991005598619307891
- Copyright
- © 2019 Informa UK Limited
- Murdoch Affiliation
- Centre for Computational and Systems Medicine
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 2 Chemistry
- 2.211 Mass Spectrometry
- 2.211.990 Metabolomics
- Web Of Science research areas
- Pharmacology & Pharmacy
- Toxicology
- ESI research areas
- Pharmacology & Toxicology