The microscopic examination of Phytophthora cinnamomi in plant tissues using fluorescent in situ hybridization

A.Y. Li; M. Crone; P.J. Adams; S.G. Fenwick; G.E.St.J. Hardy; N. Williams

doi:10.1111/jph.12257

Back

The microscopic examination of Phytophthora cinnamomi in plant tissues using fluorescent in situ hybridization

Journal article

Open access

Peer reviewed

The microscopic examination of Phytophthora cinnamomi in plant tissues using fluorescent in situ hybridization

A.Y. Li, M. Crone, P.J. Adams, S.G. Fenwick, G.E.St.J. Hardy and N. Williams

Journal of Phytopathology, Vol.162(11-12), pp.747-757

2014

DOI: https://doi.org/10.1111/jph.12257

Files and links (2)

pdf

microscopic_examination_of_Phytophthora_cinnamomi.pdfDownload View

Author’s VersionPreprint Open Access

url

Link to Published Version *Subscription may be requiredView

Abstract

The microscopic examination of Phytophthora cinnamomi in plant tissues is often difficult as structures such as hyphae, chlamydospores and oospores are frequently indistinguishable from those of other fungi and oomycetes, with histological stains not enabling species differentiation. This lack of staining specificity makes the localization of P. cinnamomi hyphae and reproductive structures within plant tissues difficult, especially in woody tissues. This study demonstrates that with the use of a species-specific fluorescently labelled DNA probe, P. cinnamomi can be specifically detected and visualized directly using fluorescent in situ hybridization (FISH) without damage to plant or pathogen cell integrity or the need for subculturing. This approach provides a new application for FISH with potential use in the detailed study of plant–pathogen interactions in plants.

Details

Title: The microscopic examination of Phytophthora cinnamomi in plant tissues using fluorescent in situ hybridization
Authors/Creators: A.Y. Li (Author/Creator)
M. Crone (Author/Creator)
P.J. Adams (Author/Creator)
S.G. Fenwick (Author/Creator)
G.E.St.J. Hardy (Author/Creator)
N. Williams (Author/Creator)
Publication Details: Journal of Phytopathology, Vol.162(11-12), pp.747-757
Publisher: Blackwell Publishing Inc.
Identifiers: 991005543914007891
Murdoch Affiliation: Centre for Phytophthora Science and Management; School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

Metrics

358 File views/ downloads

161 Record Views

6 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.97 Plant Pathology; 3.97.1439 Phytophthora Management
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science