Journal article
The microscopic examination of Phytophthora cinnamomi in plant tissues using fluorescent in situ hybridization
Journal of Phytopathology, Vol.162(11-12), pp.747-757
2014
Abstract
The microscopic examination of Phytophthora cinnamomi in plant tissues is often difficult as structures such as hyphae, chlamydospores and oospores are frequently indistinguishable from those of other fungi and oomycetes, with histological stains not enabling species differentiation. This lack of staining specificity makes the localization of P. cinnamomi hyphae and reproductive structures within plant tissues difficult, especially in woody tissues. This study demonstrates that with the use of a species-specific fluorescently labelled DNA probe, P. cinnamomi can be specifically detected and visualized directly using fluorescent in situ hybridization (FISH) without damage to plant or pathogen cell integrity or the need for subculturing. This approach provides a new application for FISH with potential use in the detailed study of plant–pathogen interactions in plants.
Details
- Title
- The microscopic examination of Phytophthora cinnamomi in plant tissues using fluorescent in situ hybridization
- Authors/Creators
- A.Y. Li (Author/Creator)M. Crone (Author/Creator)P.J. Adams (Author/Creator)S.G. Fenwick (Author/Creator)G.E.St.J. Hardy (Author/Creator)N. Williams (Author/Creator)
- Publication Details
- Journal of Phytopathology, Vol.162(11-12), pp.747-757
- Publisher
- Blackwell Publishing Inc.
- Identifiers
- 991005543914007891
- Copyright
- © 2014 Blackwell Verlag GmbH
- Murdoch Affiliation
- Centre for Phytophthora Science and Management; School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.1439 Phytophthora Management
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science