Journal article
The multiple mechanisms of Ca2+signalling by listeriolysin O, the cholesterol-dependent cytolysin of Listeria monocytogenes
Cellular Microbiology, Vol.9(8), pp.2008-2021
2007
Abstract
Cholesterol-dependent cytolysins (CDCs) represent a large family of conserved pore-forming toxins produced by several Gram-positive bacteria such as Listeria monocytogenes, Streptococcus pyrogenes and Bacillus anthracis. These toxins trigger a broad range of cellular responses that greatly influence pathogenesis. Using mast cells, we demonstrate that listeriolysin O (LLO), a prototype of CDCs produced by L. monocytogenes, triggers cellular responses such as degranulation and cytokine synthesis in a Ca2+-dependent manner. Ca2+ signalling by LLO is due to Ca2+ influx from extracellular milieu and release of from intracellular stores. We show that LLO-induced release of Ca2+ from intracellular stores occurs via at least two mechanisms: (i) activation of intracellular Ca2+ channels and (ii) a Ca2+ channels independent mechanism. The former involves PLC-IP3R operated Ca2+ channels activated via G-proteins and protein tyrosine kinases. For the latter, we propose a novel mechanism of intracellular Ca2+ release involving injury of intracellular Ca2+ stores such as the endoplasmic reticulum. In addition to Ca2+ signalling, the discovery that LLO causes damage to an intracellular organelle provides a new perspective in our understanding of how CDCs affect target cells during infection by the respective bacterial pathogens.
Details
- Title
- The multiple mechanisms of Ca2+signalling by listeriolysin O, the cholesterol-dependent cytolysin of Listeria monocytogenes
- Authors/Creators
- N.O. Gekara (Author/Creator) - Helmholtz Centre for Infection ResearchK. Westphal (Author/Creator) - Center of Molecular Immunology (Cuba)B. Ma (Author/Creator) - Institute of Molecular BiotechnologyM. Rohde (Author/Creator) - Microbiology andL. Groebe (Author/Creator) - Helmholtz Centre for Infection ResearchS. Weiss (Author/Creator) - Center of Molecular Immunology (Cuba)
- Publication Details
- Cellular Microbiology, Vol.9(8), pp.2008-2021
- Publisher
- Blackwell Publishing Ltd.
- Identifiers
- 991005544978007891
- Copyright
- © 2007 Blackwood Publishing Ltd
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.42 Bacteriology
- 1.42.678 Listeria Monocytogenes
- Web Of Science research areas
- Cell Biology
- Microbiology
- ESI research areas
- Microbiology