Journal article
The nature of diversity of HLA-DRB1 exon 3
Tissue Antigens, Vol.70(4), pp.335-337
2007
Abstract
Exon 3 of DRB1 is known to be polymorphic, but thought to be conserved within allelic groups. This implies that exon 3 polymorphisms would not need to be considered in evolutionary studies or clinical settings when assessing immunogenicity of allelic mismatches in stem cell transplantation. To further assess this, we determined the sequences of DRB1 exon 3 by hemizygote amplification and direct sequencing on 55 selected DNA samples containing 42 DRB1 alleles for which no exon 3 sequence data were previously available. The data confirmed the high degree of overall sequence conservation. The DRB4- and DRB5-associated alleles were completely conserved within their DRB1 groups. However, it could be shown that exon 3 is more diverse than previously expected. Multiple allelic differences within each group of DRB3-associated DRB1 alleles were found, without identifying unique group-related sequence motifs differentiating between these groups. For DRB1*1402 and DRB1*1406, it could be shown that they originated from DRB1*0302. In several samples previously typed as DRB1*1401, a novel DRB1 allele was identified: DRB1*1454. Thus, from a clinical viewpoint, the availability of exon 3 sequence information may be useful for optimizing typing as well as matching strategies. Additionally, it will allow for more detailed evolutionary studies, further elucidating the origin of alleles and the mechanisms driving sequence diversification.
Details
- Title
- The nature of diversity of HLA-DRB1 exon 3
- Authors/Creators
- P. A. Horn (Author/Creator) - Medizinische Hochschule HannoverM. Albis-Camps (Author/Creator) - Institute for Transfusion MedicineM. Verboom (Author/Creator) - Institute for Transfusion MedicineM. Bunce (Author/Creator) - STR Biotech (South Korea)K. Yousaf (Author/Creator) - STR Biotech (South Korea)S. Williams (Author/Creator) - STR Biotech (South Korea)R. Blasczyk (Author/Creator) - Institute for Transfusion Medicine
- Publication Details
- Tissue Antigens, Vol.70(4), pp.335-337
- Publisher
- Blackwell Publishing
- Identifiers
- 991005545548007891
- Copyright
- © 2007 Blackwell Munksgaard
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.607 MHC Diversity
- Web Of Science research areas
- Cell Biology
- Immunology
- Pathology
- ESI research areas
- Molecular Biology & Genetics