The response of intact Strongyloides ratti infective (L3) larvae to substrates and inhibitors of respiratory electron transport

A.H.W. Mendis; A. Armson; R.C.A. Thompson; W.B. Grubb

doi:10.1016/0020-7519(91)90174-6

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The response of intact Strongyloides ratti infective (L3) larvae to substrates and inhibitors of respiratory electron transport

Journal article

Peer reviewed

The response of intact Strongyloides ratti infective (L3) larvae to substrates and inhibitors of respiratory electron transport

A.H.W. Mendis, A. Armson, R.C.A. Thompson and W.B. Grubb

International Journal for Parasitology, Vol.21(8), pp.965-968

1991

DOI: https://doi.org/10.1016/0020-7519(91)90174-6

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Abstract

Live, intact third-stage larvae (L3s) of Strongyloides ratti in the absence of exogenous substrates consumed oxygen at a rate (E-QO2) of 181.8 ± 12.4 ng atoms min-1 mg dry weight-1 at 35°C. Respiratory electron transport (RET) Complex I inhibitor rotenone (2 μM) produced 33 ± 6.5% inhibition of the E-QO2. Unusually the rotenone-induced inhibition was not relieved by 5 mM-succinate. The E-QO2 of intact L3s was refractory to RET Complex III inhibitor antimycin A at 2 μM; 4 μM-antimycin inhibited ≤10% of the E-QO2. The electron donor couple ascorbate/TMPD augmented the E-QO2 in the presence of rotenone (2 μM) and antimycin A (4 μM) by 110%. Azide (1 mM) stimulated the antimycin A refractory QO2 by 36.6 ± 7.2% which was only partially inhibited by 1.0 mM-KCN (IC50 = 0.8 mM). The data suggest the presence of classical (CPW) and alternate (APW) electron transport pathways in S. ratti L3s.

Details

Title: The response of intact Strongyloides ratti infective (L3) larvae to substrates and inhibitors of respiratory electron transport
Authors/Creators: A.H.W. Mendis (Author/Creator) - Curtin University
A. Armson (Author/Creator) - Curtin University
R.C.A. Thompson (Author/Creator) - Murdoch University
W.B. Grubb (Author/Creator) - Curtin University
Publication Details: International Journal for Parasitology, Vol.21(8), pp.965-968
Publisher: Elsevier BV
Identifiers: 991005546004407891
Murdoch Affiliation: School of Veterinary Studies
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.163 Parasitology - General; 1.163.1022 Anthelmintic Resistance
Web Of Science research areas: Parasitology
ESI research areas: Microbiology