Journal article
The role of autophagy during murine primordial follicle assembly
Aging, Vol.10(2), pp.197-211
2018
Abstract
It is generally accepted that significant germ cell loss occurs during the establishment of the primordial follicle pool in most mammalian ovaries around the time of birth. However, the underlying mechanisms responsible for these processes remain largely unknown. In this investigation, we explored the role of autophagy during the establishment of the primordial follicle pool and found that autophagy was active in this process. Our data suggested that 17.5 dpc ovaries treated with rapamycin displayed a delay in germ cell cyst breakdown resulting in more oocytes at day 5 of treatment, while, ovaries that treated with 3-MA showed the opposite effect. We found that rapamycin treatment promoted autophagy and depressed cell apoptosis increasing the number of NOBOX positive oocytes. Furthermore, our results also revealed that epigenetic regulator, Sirt1, plays a role in germ cell loss. An epigenetic inhibitor or RNAi treatment of Sirt1, showed an increased level of H4K16ac and a decreased level of autophagy. Thus, these data indicate that autophagy prevents germ cell over loss during the establishment of primordial follicle pool, and this process may be influenced by Sirt1-invovled epigenetic regulation.
Details
- Title
- The role of autophagy during murine primordial follicle assembly
- Authors/Creators
- Y-C Sun (Author/Creator) - Northwest A&F UniversityY-Y Wang (Author/Creator) - Qingdao UniversityX-F Sun (Author/Creator) - Qingdao Agricultural UniversityS-F Cheng (Author/Creator) - Qingdao Agricultural UniversityL. Li (Author/Creator) - Qingdao Agricultural UniversityY. Zhao (Author/Creator) - Qingdao Agricultural UniversityW. Shen (Author/Creator) - Qingdao Agricultural UniversityH. Chen (Author/Creator) - Northwest A&F University
- Publication Details
- Aging, Vol.10(2), pp.197-211
- Publisher
- Impact Journals
- Identifiers
- 991005544081907891
- Copyright
- © 2022 Sun et al.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.81 Reproductive Biology
- 1.81.1408 Fertility Preservation
- Web Of Science research areas
- Cell Biology
- Geriatrics & Gerontology
- ESI research areas
- Molecular Biology & Genetics