The structure of Mordacia mordax insulin supports the monophyly of the Petromyzontiformes and an ancient divergence of Mordaciidae and Geotriidae

J.M. Conlon; Y. Wang; I.C. Potter

doi:10.1016/S1096-4959(00)00365-1

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The structure of Mordacia mordax insulin supports the monophyly of the Petromyzontiformes and an ancient divergence of Mordaciidae and Geotriidae

Journal article

Peer reviewed

The structure of Mordacia mordax insulin supports the monophyly of the Petromyzontiformes and an ancient divergence of Mordaciidae and Geotriidae

J.M. Conlon, Y. Wang and I.C. Potter

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Vol.129(1), pp.65-71

2001

DOI: https://doi.org/10.1016/S1096-4959(00)00365-1

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Abstract

The phylogenetic relationships between the two southern hemisphere lamprey families (Geotriidae and Mordaciidae) and their northern hemisphere counterparts (Petromyzontidae) are unresolved. Insulin was isolated from an extract of islet-containing intestinal tissue from ammocoetes of the Australian lamprey, Mordacia mordax. Its primary structure was established as A-chain: GIVEQCCHRK10CSIYDMENYC20N and B-chain: SALMGTGGTH10LCGSHLVEAL20YVVCGQRGFF30 YTP[SKG]. Although the residues in parentheses are only tentatively assigned, mass spectrometry supports the proposed sequence and demonstrates that Mordacia proinsulin, unlike proinsulin from Geotria australis, is fully processed to mature insulin. Insulins from M. mordax and G. australis and from the northern hemisphere lampreys Petromyzon marinus and Lampetra fluviatilis share a pentapeptide extension to N-terminus of the B-chain (Ser-Ala-Leu-Xaa-Gly) that has never been found in the insulins of any other vertebrate class. This observation provides support for the claim that the Petromyzontiformes constitute a monophyletic group. M. mordax insulin differs from that of G. australis by 18 amino acid residues but by only four residues from the common sequence of P. marinus and L. fluviatilis insulin. These data are consistent with the view that Geotriidae and Mordaciidae have been separated for a long period and suggest that G. australis insulin has undergone an accelerated rate of molecular evolution.

Details

Title: The structure of Mordacia mordax insulin supports the monophyly of the Petromyzontiformes and an ancient divergence of Mordaciidae and Geotriidae
Authors/Creators: J.M. Conlon (Author/Creator) - Creighton University
Y. Wang (Author/Creator) - Creighton University
I.C. Potter (Author/Creator) - Murdoch University
Publication Details: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Vol.129(1), pp.65-71
Publisher: Elsevier BV
Identifiers: 991005544404707891
Copyright: © 2001 Elsevier Science Inc.
Murdoch Affiliation: School of Biological Sciences and Biotechnology
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.26 Diabetes; 1.26.1099 Insulin Signaling
Web Of Science research areas: Biochemistry & Molecular Biology; Zoology
ESI research areas: Biology & Biochemistry