Files and links (1)
CC BY V4.0, Open Access
Journal article
The study of structure and effects of two new proanthocyanidins from Anogeissus pendula leaves on rumen enzyme activities
Frontiers in veterinary science, Vol.10, Art. 1163197
2023
Abstract
Two novel proanthocyanidins, (2R, 3R)-(+)-Gallocatechin-(4β → 8)4-(2R, 3R)-(+)-gallocatechin (compound 1) and 3-O-galloyl-(2S, 3S)-(–)-epicatechin-(4α → 8)-[3-O-galloyl-(2S, 3S)-(–)-epicatechin (4α → 8)]2-(2S, 3S)-(–)-epicatechin (compound 2), were structurally characterized from leaves of Anogeissus pendula. The structures were determined by ultraviolet spectroscopy (UV), proton nuclear magnetic resonance (1H NMR), 13C NMR, and heteronuclear multiple bond correlation. Molinspiration and Osiris property explorer applications were used to predict bioactivity and drug score. Drug scores of 0.08 and 0.05 were predicted for compounds 1 and 2, respectively. Predicted bioactivity scores were high. Due to their molecular weight, chemical structure, and conformation, the newly discovered proanthocyanidins possess an inclination to interact with proteins. Based on this premise, both compounds were subjected to in vitro testing against ruminal enzymes. They exhibited significant inhibition activities (p < 0.01) with a range of half maximal effective concentration (EC50) of 14.80–17.88 mg/mL of glutamic oxaloacetic transaminase in both protozoa and bacteria fractions. The ruminal glutamic pyruvic transaminase activity was significantly inhibited (p < 0.01) from EC50 12.59–16.29 mg/mL, and R-cellulase inhibition was recorded with EC50 18.20–21.98 mg/mL by compounds 1 and 2, respectively. Protease activity decreased with increasing incubation time and concentration of both compounds. The novel proanthocyanidins have potential roles in improving feed conversion ratios and in drug development.
Details
- Title
- The study of structure and effects of two new proanthocyanidins from Anogeissus pendula leaves on rumen enzyme activities
- Authors/Creators
- Suman Lata - Plant Animal Relationship, Indian Council of Agricultural Research (ICAR)-Indian Grassland and Fodder Research Institute, Jhansi, IndiaPushpendra Koli - Murdoch UniversityPushpendra Koli - Murdoch UniversitySultan Singh - Indian Grassland and Fodder Research InstituteB. K. Bhadoria - Plant Animal Relationship, Indian Council of Agricultural Research (ICAR)-Indian Grassland and Fodder Research Institute, Jhansi, IndiaUmesh Chand - Central University of PunjabYonglin Ren - Murdoch University, Centre for Biosecurity and One Health
- Publication Details
- Frontiers in veterinary science, Vol.10, Art. 1163197
- Publisher
- Frontiers Media S.A
- Identifiers
- 991005571669207891
- Copyright
- © 2023 Lata et al.
- Murdoch Affiliation
- Centre for Biosecurity and One Health
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
71 File views/ downloads
46 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.51 Dairy & Animal Sciences
- 3.51.84 Ruminant Nutrition
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science