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The survival of Ostertagia circumcincta and Trichostrongylus colubriformis in faecal culture as a source of bias in apportioning egg counts to worm species
Journal article   Peer reviewed

The survival of Ostertagia circumcincta and Trichostrongylus colubriformis in faecal culture as a source of bias in apportioning egg counts to worm species

R.J. Dobson, E.H. Barnes, S.D. Birclijin and J.H. Gill
International Journal for Parasitology, Vol.22(7), pp.1005-1008
1992
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Abstract

When cultured alone or concurrently with Trichostrongylus colubriformis in sheep faeces, Ostertagia circumcincta produced fewer infective larvae per 100 eggs than did T. colubriformis. Averaged over five trials 60% of T. colubriformis eggs were recovered as infective larvae while for O. circumcincta the figure was only 39%. This result was observed for two strains of O. circumcincta and was independent of when larvae were harvested from culture (days 6-10 at 25°C). The mortalities of both species occurred at the first and second larval stages. These observations are of concern when using larval differentiation from faecal culture to make quantitative estimates of worm egg numbers for each species present. Species such as T. colubriformis which have a low mortality during culture are likely to have their egg numbers overestimated when cultured with a species, like O. circumcincta, that suffers high mortality in culture.

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Citation topics
1 Clinical & Life Sciences
1.163 Parasitology - General
1.163.1022 Anthelmintic Resistance
Web Of Science research areas
Parasitology
ESI research areas
Microbiology
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