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The transport and catabolism of L-proline by cowpea Rhizobium NGR 234
Journal article   Peer reviewed

The transport and catabolism of L-proline by cowpea Rhizobium NGR 234

A.R. Glenn, S. Holliday and M.J. Dilworth
FEMS Microbiology Letters, Vol.82(3), pp.307-312
1991
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Abstract

Cowpea Rhizobium NGR234 grows with a mean generation time of 3.5 h on L-proline; intracellular catabolism via proline oxidase and pyrroline 5-carboxylate dehydrogenase is induced by proline. In contrast, cells have some capacity to take up proline whatever the growth medium. Uptake of proline is active and transmembrane gradients of approximately 290-fold can be generated. The proline transporter(s) appear to carry a range of amino acids and intracellular [14C]proline can be exchanged with extracellular valine or histidine or isoleucine but not glutamate. Snake bean bacteroids are unable to transport or catabolize L-proline, consistent with the lack of detectable proline in snakebean nodule cytosol. In this symbiosis at least, proline is not an important carbon source for bacteroids.

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