The use of a hypo-osmotic swelling (HOS) test on sperm of the pig (Sus scrofa domesticus), emu (Dromaius novaehollandiae), Asian elephant (Elephas maximus), hamadryas baboon (Papio hamadryas hamadryas), and central rock rat (Zyzomys pedunculatus)

P. Matson; W Kappelle; I. Malecki

doi:10.1016/S1642-431X(12)60025-4

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The use of a hypo-osmotic swelling (HOS) test on sperm of the pig (Sus scrofa domesticus), emu (Dromaius novaehollandiae), Asian elephant (Elephas maximus), hamadryas baboon (Papio hamadryas hamadryas), and central rock rat (Zyzomys pedunculatus)

Journal article

Peer reviewed

The use of a hypo-osmotic swelling (HOS) test on sperm of the pig (Sus scrofa domesticus), emu (Dromaius novaehollandiae), Asian elephant (Elephas maximus), hamadryas baboon (Papio hamadryas hamadryas), and central rock rat (Zyzomys pedunculatus)

P. Matson, W Kappelle and I. Malecki

Reproductive Biology, Vol.9(2), pp.181-187

2009

DOI: https://doi.org/10.1016/S1642-431X(12)60025-4

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Abstract

hypo-osmotic swelling test

sperm viability

mammals

emu

A hypo-osmotic swelling test using TALP-HEPES medium over a range of 50 to 300 mOsm/kg was applied to sperm from domestic and endangered species. Maximal responses of curling of the sperm tails were seen over a range of osmolalities for epididymal sperm from the pig (100 mOsm/kg), hamadryas baboon (range 50-125 mOsm/kg), and central rock rat (range 50-100 mOsm/kg), and the ejaculated sperm from the emu (50 mOsm/kg) and the Asian elephant (range 75-150 mOsm/kg). A solution of TALP-HEPES medium at 100 mOsm/kg would be suitable to obtain the maximal response in this range of mammals tested, though it would need to be diluted to at least 50 mOsm/kg when testing the viability of the emu sperm.

Details

Title: The use of a hypo-osmotic swelling (HOS) test on sperm of the pig (Sus scrofa domesticus), emu (Dromaius novaehollandiae), Asian elephant (Elephas maximus), hamadryas baboon (Papio hamadryas hamadryas), and central rock rat (Zyzomys pedunculatus)
Authors/Creators: P. Matson (Author/Creator)
W Kappelle (Author/Creator)
I. Malecki (Author/Creator)
Publication Details: Reproductive Biology, Vol.9(2), pp.181-187
Publisher: Polish Society for Biology of Reproduction
Number of pages: 7
Identifiers: 991005544956107891
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.81 Reproductive Biology; 1.81.176 Male Fertility
Web Of Science research areas: Reproductive Biology
ESI research areas: Clinical Medicine