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The use of activities of carbon catabolic enzymes as a probe for the carbon nutrition of Snakebean nodule bacteroids
Journal article   Peer reviewed

The use of activities of carbon catabolic enzymes as a probe for the carbon nutrition of Snakebean nodule bacteroids

S. Saroso, M.J. Dilworth and A.R. Glenn
Microbiology, Vol.132(2), pp.243-249
1986
DOI: https://doi.org/10.1099/00221287-132-2-243
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Abstract

Summary: In sugar-grown cells of cowpea Rhizobium strain NGR234 activities for enzymes of the Entner-Doudoroff and pentose phosphate pathways were present while the virtual absence of phospho-fructokinase and fructose-bisphosphate aldolase indicated that the Embden–Meyerhof–Parnas pathway was unlikely to be significant. Invertase, fructokinase, glucose-6-phosphate dehydrogenase and the Entner–Doudoroff enzymes were present at only low activities in succinate grown cells, but were induced in sugar-grown cells. Isolated snakebean bacteroids contained very low activities of these four enzymes. Although C4-dicarboxylic acids exerted some repressive effect on induction of these enzymes, there was substantial enzyme activity induced in cells grown on sucrose plus a C4 dicarboxylic acid. The data suggest that the peribacteroid membrane may be relatively impermeable to sugars and so dictate the carbon source(s) available to the bacteroids.

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