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Theory of microbial carbonate precipitation and its application in restoration of cement-based materials defects
Journal article   Peer reviewed

Theory of microbial carbonate precipitation and its application in restoration of cement-based materials defects

C. Qian, R. Wang, L. Cheng and J. Wang
Chinese Journal of Chemistry, Vol.28(5), pp.847-857
2010
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Abstract

Bacterial induced carbonate mineralization has been demonstrated as a new potential method for restoration of limestones in historic buildings and monuments. We claim here the formation of calcium carbonate was controlled by extracellular polymeric substances (EPS) isolated from Bacillus pasteurii. The process of crystallization nucleation was accelerated in the presence of cells and inhibited in the presence of EPS. The CaCO3 film deposited on cement paste surface was about 100 μm after 7 d treatment. The results of various restoring methods showed that higher decrease of water absorption of cement paste was gained in brushing application in the presence of agar, which could maintain urease with high activity in long term compared to spraying method. The coefficient of capillary suction of cement paste treated with brushing method was reduced by 90%. Mixed media consisted of sands, urea, Ca2+ and concentrated biomass, was injected into artificial cracks of cement paste followed by continual nutrient supplement, and CaCO3 particles were precipitated gradually between sands particles which were combined with cement matrix. The results showed that the compressive strength of recovered specimens was restored to 84%, which demonstrated that this kind of bio-restoration method is effective in repairing surface defects of cement-based materials.

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Citation topics
7 Engineering & Materials Science
7.133 Geotechnical Engineering
7.133.2345 MICP
Web Of Science research areas
Chemistry, Multidisciplinary
ESI research areas
Chemistry
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