Tissue inhibitor of metalloproteinases 1, a novel biomarker of tuberculosis

Y. Chen; J. Wang; P. Ge; D. Cao; B. Miao; I. Robertson; X. Zhou; L. Zhang; H. Chen; A. Guo

doi:10.3892/mmr.2016.5998

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Tissue inhibitor of metalloproteinases 1, a novel biomarker of tuberculosis

Journal article

Open access

Peer reviewed

Tissue inhibitor of metalloproteinases 1, a novel biomarker of tuberculosis

Y. Chen, J. Wang, P. Ge, D. Cao, B. Miao, I. Robertson, X. Zhou, L. Zhang, H. Chen and A. Guo

Molecular Medicine Reports, Vol.15(1), pp.483-487

2017

DOI: https://doi.org/10.3892/mmr.2016.5998

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Abstract

Tuberculosis (TB) is an important infectious disease of humans and other animals. Conventional diagnostic methods, including the tuberculin skin test, chest X-rays and bacterial culture, have certain innate disadvantages for the early, rapid and specific diagnosis of tuberculosis. The present study aimed to identify a novel diagnostic biomarker to overcome these disadvantages. The potential target identified in the present study was tissue inhibitor of metalloproteinases 1 (TIMP-1), which has previously been demonstrated to be critical in the immune response to TB. The concentration of TIMP-1 in the blood was determined using a commercial ELISA kit, and the relative mRNA expression levels following bacterial infection were detected by reverse transcription-quantitative polymerase chain reaction. Based on a clinical and microbiological diagnosis, the ELISA for plasma TIMP-1 had a sensitivity of 91.80% [95% confidence interval (CI): 85.44, 96.00] and a specificity of 91.41% (95% CI: 85.14, 95.63). In a THP-1 cell model, Bacillus Calmette-Guérin and Mycobacterium bovis significantly upregulated the mRNA expression levels of TIMP-1 post infection in a time-dependent manner (P=0.006 for BCG 24 h PI, P=3.2x10-7 for M. bovis 24 PI). The results of the present study indicate that plasma TIMP-1 may be a potential biomarker for the diagnosis of TB.

Details

Title: Tissue inhibitor of metalloproteinases 1, a novel biomarker of tuberculosis
Authors/Creators: Y. Chen (Author/Creator) - Huazhong Agricultural University
J. Wang (Author/Creator) - Huazhong Agricultural University
P. Ge (Author/Creator) - Huazhong Agricultural University
D. Cao (Author/Creator) - Huazhong Agricultural University
B. Miao (Author/Creator) - Huazhong Agricultural University
I. Robertson (Author/Creator) - China Australia Joint Research and Training Center for Veterinary Epidemiology, Wuhan, Hubei 430070, P.R. China.
X. Zhou (Author/Creator) - Wuhan Pulmonary Hospital
L. Zhang (Author/Creator) - Wuhan Pulmonary Hospital
H. Chen (Author/Creator) - Huazhong Agricultural University
A. Guo (Author/Creator) - Huazhong Agricultural University
Publication Details: Molecular Medicine Reports, Vol.15(1), pp.483-487
Publisher: Spandidos Publications
Identifiers: 991005541954707891
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.194 Tuberculosis & Leprosy; 1.194.273 TB Diagnostics & Treatment
Web Of Science research areas: Medicine, Research & Experimental; Oncology
ESI research areas: Clinical Medicine