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Tissue oxygen tension and blood-flow changes in rat incisor pulp with graded systemic hyperoxia
Journal article   Peer reviewed

Tissue oxygen tension and blood-flow changes in rat incisor pulp with graded systemic hyperoxia

C.Y. Yu, N.M. Boyd, S.J. Cringle, V.A. Alder and D-Y Yu
Archives of Oral Biology, Vol.47(3), pp.239-246
2002
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Abstract

The role of oxygen in the regulation of the pulpal microcirculation is unknown. This investigation is aimed to measure tissue oxygen tension and blood-flow changes in the pulp of rat lower incisors during graded systemic hyperoxia, and to determine the response of the pulpal vasculature to various oxygen tensions. Twenty-four Sprague-Dawley rats were anaesthetized and artificially ventilated with the appropriate gas mixture. Recessed oxygen-sensitive microelectrodes were used to measure pulpal tissue oxygen tension via a small access cavity filled with saline on the labial surface of the incisor. A laser Doppler flowmeter was used to record pulpal blood-flow. Inspired oxygen was increased stepwise from 20 to 100% in 20% steps. Systemic blood-gas concentrations were measured at each step. Systemic arterial oxygen tension at 100% oxygen ventilation reached 481.2 ± 30.7% of the baseline at 20% oxygen breathing (n = 21). Pulpal tissue oxygen tension did not change significantly whereas pulpal blood-flow fell dose-dependently to 74.6 ± 5.0% at 100% oxygen ventilation (n = 21). Systemic hyperoxia, therefore, induces a significant reduction in pulpal blood-flow whereas pulpal tissue oxygen tension remains relatively stable, indicating an oxygen-dependent local regulatory mechanism.

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Collaboration types
Domestic collaboration
Citation topics
1 Clinical & Life Sciences
1.195 Neuroendocrine & Intestinal Disorders
1.195.578 Neuropeptide Roles
Web Of Science research areas
Dentistry, Oral Surgery & Medicine
ESI research areas
Clinical Medicine
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