Journal article
Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T Cells
Transplantation, Vol.91(5), pp.494-500
2011
Abstract
Background: The crossreactivity of Epstein-Barr virus (EBV Epstein-Barr virus nuclear antigen 3A [EBNA3A])-specific CD8 T cells against allogeneic human leukocyte antigen (HLA)-B*44:02 has been shown to be dependent on presentation of self-peptide EEYLQAFTY by the target antigen. In this study, we report that allogeneic HLA-B*44:02+ proximal tubular epithelial cells (PTECs) and human umbilical vein endothelial cells (HUVECs) are poor targets for EBV EBNA3A-specific T cells.
Methods: The EEY peptide was exogenously loaded onto HLA-B*44:02 and HLA-B*44:03-expressing PTECs and HUVECs. EEY-peptide-loaded, and unloaded, PTECs and HUVECs were then incubated with serial dilutions of our EBNA3A T-cell clone, in a cytotoxicity assay.
Results: Although HLA-B*44:02-expressing PTECs were specifically lysed in proportion to the effector/target ratio by the EBNA3A T-cell clone, without peptide loading, lysis was greatly increased by exogenous EEY peptide loading (15% vs. 75%; P<0.0001). HLA-B*44:02-expressing HUVECs were only lysed when loaded with exogenous EEY peptide (0% vs. 64%; P<0.0001). Lack of HLA expression and lack of ABCD3 gene expression were excluded as a cause for these results. PTECs and HUVECs were specifically targeted by another alloreactive T-cell clone without exogenous peptide loading, suggesting that the lack of recognition of HLA-B*44:02+ epithelial and endothelial cells by the EBV EBNA3A T-cell clone was due to lack of EEYLQAFTY peptide presentation.
Conclusions: Tissue-specific (peptide dependent) alloreactivity may have important implications for transplantation monitoring and rejection.
Details
- Title
- Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T Cells
- Authors/Creators
- L.J.A. DʼOrsogna (Author/Creator)D.L. Roelen (Author/Creator)E.M.W. van der Meer-Prins (Author/Creator)P. van der Pol (Author/Creator)M.E. Franke-van Dijk (Author/Creator)M. Eikmans (Author/Creator)J. Anholts (Author/Creator)J. Rossjohn (Author/Creator)J. McCluskey (Author/Creator)A. Mulder (Author/Creator)C. van Kooten (Author/Creator)I.I.N. Doxiadis (Author/Creator)F.H.J. Claas (Author/Creator)
- Publication Details
- Transplantation, Vol.91(5), pp.494-500
- Publisher
- Wolters Kluwer Health
- Identifiers
- 991005542954207891
- Copyright
- © 2017 Wolters Kluwer Health
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.127 T Cell Regulation
- Web Of Science research areas
- Immunology
- Surgery
- Transplantation
- ESI research areas
- Clinical Medicine