Journal article
Tracking the acetate threshold using DO-transient control during medium and high cell density cultivation of recombinant Escherichia coli in complex media
Biotechnology and Bioengineering, Vol.84(3), pp.314-323
2003
Abstract
DO-transient nutrient controllers use the dissolved oxygen signal to attempt acetate threshold tracking during fed-batch cultivation of recombinant E. coli. Here we apply DO-transient control to the production of Jembrana disease virus protein in complex Super Luria medium and compare performance against a high-limit pH-stat controller. For induction at medium cell density (harvest between 31 and 32.5 g dcw L) a total productivity of 0.27 g L h was achieved as compared to 0.24 g L h with the high-limit pH-stat. For induction at high cell density (harvest at 60 g dcw L), decreased productivity (0.12 g L h) was attributed to the effect of acetate accumulation on recombinant protein formation and a concomitant lowering of the critical growth rate. Our results suggest that complex media provides a difficult environment for the application of acetate threshold tracking DO-transient control because of difficulties in re-oxidizing acetate, and apparent localized production of acetate below the production threshold (as detected by the DO-transient controller as SPOUR crit). Configuring the DO-transient controller to avoid aggressive threshold probing is suggested as a means to improve performance and reduce acetate accumulation in complex media.
Details
- Title
- Tracking the acetate threshold using DO-transient control during medium and high cell density cultivation of recombinant Escherichia coli in complex media
- Authors/Creators
- W.A. Johnston (Author/Creator) - Hawaii Natural Energy Institute, University of Hawaii—Manoa, 1680 East West Road, POST 109, Honolulu, Hawaii 96822. telephone: 808‐956‐7337; fax: 808‐956‐2335M. Stewart (Author/Creator) - Murdoch UniversityP. Lee (Author/Creator) - Curtin UniversityM.J. Cooney (Author/Creator) - Hawaii Natural Energy Institute, University of Hawaii—Manoa, 1680 East West Road, POST 109, Honolulu, Hawaii 96822. telephone: 808‐956‐7337; fax: 808‐956‐2335
- Publication Details
- Biotechnology and Bioengineering, Vol.84(3), pp.314-323
- Publisher
- Wiley - V C H Verlag GmbbH & Co
- Identifiers
- 991005542040507891
- Copyright
- © 2003 Wiley Periodicals, Inc.
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 2 Chemistry
- 2.166 Chromatography & Electrophoresis
- 2.166.873 Protein Purification
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- ESI research areas
- Biology & Biochemistry