Transcriptional convergence after repeated duplication of an amino acid transporter gene leads to the independent emergence of the black husk/pericarp trait in barley and rice

Bo Li; Yong Jia; Le Xu; Shuo Zhang; Zhoukai Long; Rong Wang; Ying Guo; Wenying Zhang; Chunhai Jiao; Chengdao Li; Yanhao Xu

doi:10.1111/pbi.14264

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Transcriptional convergence after repeated duplication of an amino acid transporter gene leads to the independent emergence of the black husk/pericarp trait in barley and rice

Journal article

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Transcriptional convergence after repeated duplication of an amino acid transporter gene leads to the independent emergence of the black husk/pericarp trait in barley and rice

Bo Li, Yong Jia, Le Xu, Shuo Zhang, Zhoukai Long, Rong Wang, Ying Guo, Wenying Zhang, Chunhai Jiao, Chengdao Li, …

Plant biotechnology journal, Vol.22(5), pp.1282-1298

2023

DOI: https://doi.org/10.1111/pbi.14264

PMID: 38124464

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Abstract

barley

black husk/pericarp

rice

convergent evolution

melanin

Blp

The repeated emergence of the same trait (convergent evolution) in distinct species is an interesting phenomenon and manifests visibly the power of natural selection. The underlying genetic mechanisms have important implications to understand how the genome evolves under environmental challenges. In cereal crops, both rice and barley can develop black-coloured husk/pericarp due to melanin accumulation. However, it is unclear if this trait shares a common origin. Here, we fine-mapped the barley HvBlp gene controlling the black husk/pericarp trait and confirmed its function by gene silencing. The result was further supported by a yellow husk/pericarp mutant with deletion of the HvBlp gene, derived from gamma ray radiation of the wild-type W1. HvBlp encodes a putative tyrosine transporter homologous to the black husk gene OsBh4 in rice. Surprisingly, synteny and phylogenetic analyses showed that HvBlp and OsBh4 belonged to different lineages resulted from dispersed and tandem duplications, respectively, suggesting that the black husk/pericarp trait has emerged independently. The dispersed duplication (dated at 21.23 MYA) yielding HvBlp occurred exclusively in the common ancestor of Triticeae. HvBlp and OsBh4 displayed converged transcription in husk/pericarp tissues, contributing to the black husk/pericarp trait. Further transcriptome and metabolome data identified critical candidate genes and metabolites related to melanin production in barley. Taken together, our study described a compelling case of convergent evolution resulted from transcriptional convergence after repeated gene duplication, providing valuable genetic insights into phenotypic evolution. The identification of the black husk/pericarp genes in barley also has great potential in breeding for stress-resilient varieties with higher nutritional values.

Details

Title: Transcriptional convergence after repeated duplication of an amino acid transporter gene leads to the independent emergence of the black husk/pericarp trait in barley and rice
Authors/Creators: Bo Li - Ministry of Agriculture and Rural Affairs
Yong Jia - Department of Primary Industries and Regional Development
Le Xu - Yangtze University
Shuo Zhang - Ministry of Agriculture and Rural Affairs
Zhoukai Long - Yangtze University
Rong Wang - Yangtze University
Ying Guo - Ministry of Agriculture and Rural Affairs
Wenying Zhang - Yangtze University
Chunhai Jiao - Ministry of Agriculture and Rural Affairs
Chengdao Li - Murdoch University, Centre for Crop and Food Innovation
Yanhao Xu - Ministry of Agriculture and Rural Affairs
Publication Details: Plant biotechnology journal, Vol.22(5), pp.1282-1298
Publisher: Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
Grant note: Leading Talent Program in Hubei Academy of Agricultural Sciences 2021CFA064 / Hubei Outstanding Youth Fund 2021-620-000-001-01 / Hubei Agricultural Science and Technology Innovation Center Innovation Team Project HBZY2023A001 / Hubei Seed Industry High Quality Development Fund
Identifiers: 991005626669807891
Murdoch Affiliation: Western Crop Genetics Alliance
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.1969 Anthocyanin Biosynthesis
Web Of Science research areas: Biotechnology & Applied Microbiology; Plant Sciences
ESI research areas: Plant & Animal Science