Journal article
Transcriptional responses of toxigenic and atoxigenic isolates of Aspergillus flavus to oxidative stress in aflatoxin-conducive and non-conducive media
World Mycotoxin Journal, Vol.13(4), pp.443-457
2020
Abstract
Aflatoxin production by isolates of Aspergillus flavus varies, ranging from highly toxigenic to completely atoxigenic. Several mechanisms have been identified which regulate aflatoxin production including medium carbon source and oxidative stress. In recent studies, aflatoxin production has been implicated in partially ameliorating oxidative stress in A. flavus. To better understand the role of aflatoxin production in oxidative stress responses, a selection of toxigenic and atoxigenic isolates of A. flavus with moderate to high oxidative stress tolerance were exposed to increasing concentrations of H2O2 in both aflatoxin-conducive and non-conducive media. Mycelial mats were collected for global transcriptome sequencing followed by differential expression, functional prediction, and weighted co-expression analyses. Oxidative stress and medium carbon source had a significant effect on the expression of several secondary metabolite gene clusters including those for aflatoxin, aflatrem, aflavarin, cyclopiazonic acid, and kojic acid. Atoxigenic biological control isolates showed less differential expression under stress than other atoxigenic isolates suggesting expression profiles may be useful in screening. Increasing stress also resulted in regulation of SakA/Hog1 and MpkA MAP kinase signalling pathways pointing to their potential roles in regulating oxidative stress responses. Their expression was also influenced by medium carbon source. These results suggest that aflatoxin production along with that of other mycotoxins may occur as part of a concerted coping mechanism for oxidative stress and its effects in the environment. This mechanism is also regulated by availability of simple sugars and glycolytic compounds for their biosynthesis.
Details
- Title
- Transcriptional responses of toxigenic and atoxigenic isolates of Aspergillus flavus to oxidative stress in aflatoxin-conducive and non-conducive media
- Authors/Creators
- J.C. Fountain (Author/Creator) - Mississippi State UniversityA.K. Pandey (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsS.N. Nayak (Author/Creator) - University of Agricultural Sciences, DharwadP. Bajaj (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsH. Wang (Author/Creator) - University of GeorgiaV. Kumar (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsA. Chitikineni (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsH.K. Abbas (Author/Creator) - Agricultural Research ServiceB.T. Scully (Author/Creator) - U.S. Horticultural Research LaboratoryR.C. Kemerait (Author/Creator) - University of GeorgiaM.K. Pandey (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsB. Guo (Author/Creator) - Agricultural Research ServiceR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
- Publication Details
- World Mycotoxin Journal, Vol.13(4), pp.443-457
- Publisher
- Wageningen Academic Publishers
- Identifiers
- 991005543360307891
- Copyright
- © 2020 Wageningen Academic Publishers
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.198 Mycotoxins
- 3.198.400 Mycotoxin Control
- Web Of Science research areas
- Food Science & Technology
- Mycology
- Toxicology
- ESI research areas
- Plant & Animal Science