Transcriptome analyses reveal genotype- and developmental stage-specific molecular responses to drought and salinity stresses in chickpea

R. Garg; R. Shankar; B. Thakkar; H. Kudapa; L. Krishnamurthy; N. Mantri; R.K. Varshney; S. Bhatia; M. Jain

doi:10.1038/srep19228

Transcriptome analyses reveal genotype- and developmental stage-specific molecular responses to drought and salinity stresses in chickpea

R. Garg, R. Shankar, B. Thakkar, H. Kudapa, L. Krishnamurthy, N. Mantri, R.K. Varshney, S. Bhatia M. Jain

Scientific Reports, Vol.6(1), Art. 19228

2016

: https://doi.org/10.1038/srep19228

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Drought and salinity are the major factors that limit chickpea production worldwide. We performed whole transcriptome analyses of chickpea genotypes to investigate the molecular basis of drought and salinity stress response/adaptation. Phenotypic analyses confirmed the contrasting responses of the chickpea genotypes to drought or salinity stress. RNA-seq of the roots of drought and salinity related genotypes was carried out under control and stress conditions at vegetative and/or reproductive stages. Comparative analysis of the transcriptomes revealed divergent gene expression in the chickpea genotypes at different developmental stages. We identified a total of 4954 and 5545 genes exclusively regulated in drought-tolerant and salinity-tolerant genotypes, respectively. A significant fraction (~47%) of the transcription factor encoding genes showed differential expression under stress. The key enzymes involved in metabolic pathways, such as carbohydrate metabolism, photosynthesis, lipid metabolism, generation of precursor metabolites/energy, protein modification, redox homeostasis and cell wall component biogenesis, were affected by drought and/or salinity stresses. Interestingly, transcript isoforms showed expression specificity across the chickpea genotypes and/or developmental stages as illustrated by the AP2-EREBP family members. Our findings provide insights into the transcriptome dynamics and components of regulatory network associated with drought and salinity stress responses in chickpea.

: Transcriptome analyses reveal genotype- and developmental stage-specific molecular responses to drought and salinity stresses in chickpea
: R. Garg (Author/Creator) - National Institute of Plant Genome Research
R. Shankar (Author/Creator) - National Institute of Plant Genome Research
B. Thakkar (Author/Creator) - National Institute of Plant Genome Research
H. Kudapa (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
L. Krishnamurthy (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
N. Mantri (Author/Creator) - RMIT University
R.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
S. Bhatia (Author/Creator) - National Institute of Plant Genome Research
M. Jain (Author/Creator) - Jawaharlal Nehru University
: Scientific Reports, Vol.6(1), Art. 19228
: Springer Nature
: 991005539985007891
: Murdoch University
: English
: Journal article

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esploro.research.conf.research.portal.label.prefix.inciteCollaboration: research.portal.fulldisplay.incitesHighlights.institutionCollaboration; research.portal.fulldisplay.incitesHighlights.internationalCollaboration
esploro.research.conf.research.portal.label.prefix.inciteCitationTopics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.49 Plant Stress Responses
esploro.research.conf.research.portal.label.prefix.inciteWOSResearchAreas: Plant Sciences
esploro.research.conf.research.portal.label.prefix.inciteESIResearchAreas: Plant & Animal Science