Transcriptome and Allele Specificity Associated with a 3BL Locus for Fusarium Crown Rot Resistance in Bread Wheat

Jian Ma; Jiri Stiller; Qiang Zhao; Qi Feng; Colin Cavanagh; Penghao Wang; Donald Gardiner; Frédéric Choulet; Catherine Feuillet; You-Liang Zheng; Yuming Wei; Guijun Yan; Bin Han; John M. Manners; Chunji Liu

doi:10.1371/journal.pone.0113309

Back

Transcriptome and Allele Specificity Associated with a 3BL Locus for Fusarium Crown Rot Resistance in Bread Wheat

Journal article

Open access

Peer reviewed

Transcriptome and Allele Specificity Associated with a 3BL Locus for Fusarium Crown Rot Resistance in Bread Wheat

Jian Ma, Jiri Stiller, Qiang Zhao, Qi Feng, Colin Cavanagh, Penghao Wang, Donald Gardiner, Frédéric Choulet, Catherine Feuillet, You-Liang Zheng, …

PloS one, Vol.9(11), pp.e113309-e113309

2014

DOI: https://doi.org/10.1371/journal.pone.0113309

PMCID: PMC4236173

PMID: 25405461

Files and links (1)

pdf

Published1.47 MBDownload View

Published (Version of Record)CC BY V4.0, Open Access

Abstract

Genetic loci

Quantitative trait loci

Gene expression

Single nucleotide polymorphism

Fusarium

Transcription factors

Wheat

Genomics

Fusarium pathogens cause two major diseases in cereals, Fusarium crown rot (FCR) and head blight (FHB). A large-effect locus conferring resistance to FCR disease was previously located to chromosome arm 3BL (designated as Qcrs-3B) and several independent sets of near isogenic lines (NILs) have been developed for this locus. In this study, five sets of the NILs were used to examine transcriptional changes associated with the Qcrs-3B locus and to identify genes linked to the resistance locus as a step towards the isolation of the causative gene(s). Of the differentially expressed genes (DEGs) detected between the NILs, 12.7% was located on the single chromosome 3B. Of the expressed genes containing SNP (SNP-EGs) detected, 23.5% was mapped to this chromosome. Several of the DEGs and SNP-EGs are known to be involved in host-pathogen interactions, and a large number of the DEGs were among those detected for FHB in previous studies. Of the DEGs detected, 22 were mapped in the Qcrs-3B interval and they included eight which were detected in the resistant isolines only. The enrichment of DEG, and not necessarily those containing SNPs between the resistant and susceptible isolines, around the Qcrs-3B locus is suggestive of local regulation of this region by the resistance allele. Functions for 13 of these DEGs are known. Of the SNP-EGs, 28 were mapped in the Qcrs-3B interval and biological functions for 16 of them are known. These results provide insights into responses regulated by the 3BL locus and identify a tractable number of target genes for fine mapping and functional testing to identify the causative gene(s) at this QTL.

Details

Title: Transcriptome and Allele Specificity Associated with a 3BL Locus for Fusarium Crown Rot Resistance in Bread Wheat
Authors/Creators: Jian Ma - Sichuan Agricultural University
Jiri Stiller - Commonwealth Scientific and Industrial Research Organisation
Qiang Zhao - National Center for Gene Research
Qi Feng - National Center for Gene Research
Colin Cavanagh - Commonwealth Scientific and Industrial Research Organisation
Penghao Wang - Commonwealth Scientific and Industrial Research Organisation
Donald Gardiner - Commonwealth Scientific and Industrial Research Organisation
Frédéric Choulet - Génétique, Diversité, Écophysiologie des Céréales
Catherine Feuillet - Génétique, Diversité, Écophysiologie des Céréales
You-Liang Zheng - Sichuan Agricultural University
Yuming Wei - Sichuan Agricultural University
Guijun Yan - The University of Western Australia
Bin Han - National Center for Gene Research
John M. Manners - Commonwealth Scientific and Industrial Research Organisation
Chunji Liu - The University of Western Australia
Publication Details: PloS one, Vol.9(11), pp.e113309-e113309
Publisher: Public Library of Science
Identifiers: 991005569356607891
Murdoch Affiliation: Centre for Crop and Food Innovation; School of Medical, Molecular and Forensic Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Metrics

1 File views/ downloads

10 Record Views

37 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.198 Mycotoxins; 3.198.400 Mycotoxin Control
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science