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Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant
Journal article   Open access   Peer reviewed

Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant

L. Wan, Y. Lei, L. Yan, Y. Liu, M.K. Pandey, X. Wan, R.K. Varshney, J. Fang and B. Liao
BMC Plant Biology, Vol.20(1), Art. 161
2020
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Abstract

Background: Coat color determines both appearance and nutrient quality of peanut. White seed coat in peanut can enhance the processing efficiency and quality of peanut oil. An integrative analysis of transcriptomes, metabolomes and histocytology was performed on wsc mutant and its wild type to investigate the regulatory mechanisms underlying color pigmentation. Result: Metabolomes revealed flavonoids were redirected in wsc, while multi-omics analyses of wsc mutant seeds and testae uncovered WSC influenced the flavonoids biosynthesis in testa as well as suberin formation, glycolysis, the TCA cycle and amino acid metabolism. The mutation also enhanced plant hormones synthesis and signaling. Further, co-expression analysis showed that FLS genes co-expressed with MBW complex member genes. Combining tissue expression patterns, genetic analyses, and the annotation of common DEGs for these three stages revealed that three testa specific expressed candidate genes, Araip.M7RY3, Aradu.R8PMF and Araip.MHR6K were likely responsible for the white testa phenotype. WSC might be regulated expression competition between FLS and DFR by controlling hormone synthesis and signaling as well as the MBW complex. Conclusions: The results of this study therefore provide both candidate genes and novel approaches that can be applied to improve peanut with desirable seed coat color and flavonoid quality.

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Collaboration types
Domestic collaboration
International collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.4 Crop Science
3.4.1969 Anthocyanin Biosynthesis
Web Of Science research areas
Plant Sciences
ESI research areas
Plant & Animal Science
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