Transcriptome and toxin family analysis of the paralysis tick, Ixodes holocyclus

M. Rodriguez-Valle; P. Moolhuijzen; R.A. Barrero; C.T. Ong; G. Busch; T. Karbanowicz; M. Booth; R. Clark; J. Koehbach; H. Ijaz; K. Broady; K. Agnew; A.G. Knowles; M.I. Bellgard; A.E. Tabor

doi:10.1016/j.ijpara.2017.07.007

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Transcriptome and toxin family analysis of the paralysis tick, Ixodes holocyclus

Journal article

Open access

Peer reviewed

Transcriptome and toxin family analysis of the paralysis tick, Ixodes holocyclus

M. Rodriguez-Valle, P. Moolhuijzen, R.A. Barrero, C.T. Ong, G. Busch, T. Karbanowicz, M. Booth, R. Clark, J. Koehbach, H. Ijaz, …

International Journal for Parasitology, Vol.48(1), pp.71-82

2017

DOI: https://doi.org/10.1016/j.ijpara.2017.07.007

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Abstract

The Australian paralysis tick (Ixodes holocyclus) secretes neuropathic toxins into saliva that induce host paralysis. Salivary glands and viscera were dissected from fully engorged female I. holocyclus ticks collected from dogs and cats with paralysis symptoms. cDNA from both tissue samples were sequenced using Illumina HiSeq 100 bp pair end read technologies. Unique and non-redundant holocyclotoxin sequences were designated as HT2–HT19, as none were identical to the previously described HT1. Specific binding to rat synaptosomes was determined for synthetic HTs, and their neurotoxic capacity was determined by neonatal mouse assay. They induced a powerful paralysis in neonatal mice, particularly HT4 which produced rapid and strong respiratory distress in all animals tested. This is the first known genomic database developed for the Australian paralysis tick. The database contributed to the identification and subsequent characterization of the holocyclotoxin family that will inform the development of novel anti-paralysis control methods.

Details

Title: Transcriptome and toxin family analysis of the paralysis tick, Ixodes holocyclus
Authors/Creators: M. Rodriguez-Valle (Author/Creator) - The University of Queensland
P. Moolhuijzen (Author/Creator) - Murdoch University
R.A. Barrero (Author/Creator) - Murdoch University
C.T. Ong (Author/Creator) - The University of Queensland
G. Busch (Author/Creator) - The University of Queensland
T. Karbanowicz (Author/Creator) - The University of Queensland
M. Booth (Author/Creator) - The University of Queensland
R. Clark (Author/Creator) - The University of Queensland
J. Koehbach (Author/Creator) - The University of Queensland
H. Ijaz (Author/Creator) - The University of Queensland
K. Broady (Author/Creator) - University of Technology Sydney
K. Agnew (Author/Creator) - Elanco (United States)
A.G. Knowles (Author/Creator) - Elanco (United States)
M.I. Bellgard (Author/Creator) - Murdoch University
A.E. Tabor (Author/Creator) - Murdoch University
Publication Details: International Journal for Parasitology, Vol.48(1), pp.71-82
Publisher: Elsevier BV
Identifiers: 991005540133907891
Copyright: © 2017 Australian Society for Parasitology
Murdoch Affiliation: Centre for Comparative Genomics
Language: English
Resource Type: Journal article

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Collaboration types: Industry collaboration; Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.258 Zoonotic Diseases; 1.258.227 Tick-borne Pathogens
Web Of Science research areas: Parasitology
ESI research areas: Microbiology