Files and links (2)
Published (Version of Record)CC BY V4.0, Open Access
Journal article
Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection
PLoS ONE, Vol.12(11), Art. e0187735
2017
Abstract
The porcine reproductive and respiratory syndrome (PRRS) is an infectious disease that leads to high financial and production losses in the global swine industry. The pathogenesis of this disease is dependent on a multitude of factors, and its control remains problematic. The immune system generally defends against infectious diseases, especially dendritic cells (DCs), which play a crucial role in the activation of the immune response after viral infections. However, the understanding of the immune response and the genetic impact on the immune response to PRRS virus (PRRSV) remains incomplete. In light of this, we investigated the regulation of the host immune response to PRRSV in porcine lung DCs using RNA-sequencing (RNA-Seq). Lung DCs from two different pig breeds (Pietrain and Duroc) were collected before (0 hours) and during various periods of infection (3, 6, 9, 12, and 24 hours post infection (hpi)). RNA-Seq analysis revealed a total of 20,396 predicted porcine genes, which included breed-specific differentially expressed immune genes. Pietrain and Duroc infected lung DCs showed opposite gene expression courses during the first time points post infection. Duroc lung DCs reacted more strongly and distinctly than Pietrain lung DCs during these periods (3, 6, 9, 12 hpi). Additionally, cluster analysis revealed time-dependent co-expressed groups of genes that were involved in immune-relevant pathways. Key clusters and pathways were identified, which help to explain the biological and functional background of lung DCs post PRRSV infection and suggest IL-1β1 as an important candidate gene. RNA-Seq was also used to characterize the viral replication of PRRSV for each breed. PRRSV was able to infect and to replicate differently in lung DCs between the two mentioned breeds. These results could be useful in investigations on immunity traits in pig breeding and enhancing the health of pigs.
Details
- Title
- Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection
- Authors/Creators
- M.J. Pröll (Author/Creator) - University of BonnC. Neuhoff (Author/Creator) - University of BonnK. Schellander (Author/Creator) - University of BonnM.J. Uddin (Author/Creator) - The University of QueenslandM.U. Cinar (Author/Creator) - Erciyes UniversityS. Sahadevan (Author/Creator) - University of CopenhagenX. Qu (Author/Creator) - Shenzhen Institutes of Advanced TechnologyMd.A. Islam (Author/Creator) - University of BonnM. Poirier (Author/Creator) - University of BonnM.A. Müller (Author/Creator) - Charité - Universitätsmedizin BerlinC. Drosten (Author/Creator) - Charité - Universitätsmedizin BerlinD. Tesfaye (Author/Creator) - University of BonnE. Tholen (Author/Creator) - University of BonnC. Große-Brinkhaus (Author/Creator) - University of Bonn
- Publication Details
- PLoS ONE, Vol.12(11), Art. e0187735
- Publisher
- Public Library of Science
- Identifiers
- 991005542615807891
- Copyright
- © 2017 ProÈll et al.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
13 File views/ downloads
44 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.104 Virology - General
- 1.104.1933 Porcine Viral Challenges
- Web Of Science research areas
- Multidisciplinary Sciences
- ESI research areas
- Multidisciplinary