Journal article
Transcriptome reprogramming due to the introduction of a barley telosome into bread wheat affects more barley genes than wheat
Plant Biotechnology Journal, Vol.16(10), pp.1767-1777
2018
Abstract
Despite a long history, the production of useful alien introgression lines in wheat remains difficult mainly due to linkage drag and incomplete genetic compensation. In addition, little is known about the molecular mechanisms underlying the impact of foreign chromatin on plant phenotype. Here, a comparison of the transcriptomes of barley, wheat and a wheat–barley 7HL addition line allowed the transcriptional impact both on 7HL genes of a non‐native genetic background and on the wheat gene complement as a result of the presence of 7HL to be assessed. Some 42% (389/923) of the 7HL genes assayed were differentially transcribed, which was the case for only 3% (960/35 301) of the wheat gene complement. The absence of any transcript in the addition line of a suite of chromosome 7A genes implied the presence of a 36 Mbp deletion at the distal end of the 7AL arm; this deletion was found to be in common across the full set of Chinese Spring/Betzes barley addition lines. The remaining differentially transcribed wheat genes were distributed across the whole genome. The up‐regulated barley genes were mostly located in the proximal part of the 7HL arm, while the down‐regulated ones were concentrated in the distal part; as a result, genes encoding basal cellular functions tended to be transcribed, while those encoding specific functions were suppressed. An insight has been gained into gene transcription in an alien introgression line, thereby providing a basis for understanding the interactions between wheat and exotic genes in introgression materials.
Details
- Title
- Transcriptome reprogramming due to the introduction of a barley telosome into bread wheat affects more barley genes than wheat
- Authors/Creators
- E. Reyneke (Author/Creator)M. Abrouk (Author/Creator) - Czech Academy of Sciences, Institute of Experimental BotanyG. Keeble-Gagnère (Author/Creator) - Agriculture VictoriaM. Karafiátová (Author/Creator) - Czech Academy of Sciences, Institute of Experimental BotanyJ. Vrána (Author/Creator) - Czech Academy of Sciences, Institute of Experimental BotanyS. Balzergue (Author/Creator) - Université Paris-SaclayL. Soubigou-Taconnat (Author/Creator) - Université Paris-SaclayV. Brunaud (Author/Creator) - Université Paris-SaclayM-L Martin-Magniette (Author/Creator) - Institut des Sciences des Plantes de Paris SaclayT.R. Endo (Author/Creator) - Ryukoku UniversityJ. Bartoš (Author/Creator) - Czech Academy of Sciences, Institute of Experimental BotanyR. Appels (Author/Creator) - Murdoch UniversityJ. Doležel (Author/Creator) - Murdoch University
- Publication Details
- Plant Biotechnology Journal, Vol.16(10), pp.1767-1777
- Publisher
- Blackwell Publishing Inc.
- Identifiers
- 991005543429607891
- Copyright
- © 2018 The Authors
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Plant Sciences
- ESI research areas
- Plant & Animal Science