Journal article
Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron
Journal of Hepatology, Vol.48(2), pp.327-334
2008
Abstract
Background/Aims: Transferrin receptor 2 appears to have dual roles in iron metabolism; one is signalling, the other is iron transport. It is sensitive to high levels of diferric transferrin, which is associated with disorders of iron overload. Also present in these disorders are increased levels of plasma non-transferrin-bound iron. This study sought to clarify the role of transferrin receptor 2 in the uptake of transferrin-bound and non-transferrin-bound iron. Methods: Variant Chinese Hamster Ovary (CHO) cells, transfected with transferrin receptor 2, were incubated with radio-labelled transferrin-bound or non-transferrin-bound iron. Competition studies were performed in the presence of unlabelled dimetallic transferrin; knockdown was performed using specific siRNA. Results: Cells expressing transferrin receptor 2 bound and internalised transferrin and transferrin-bound iron. Transferrin recycling occurred with an average cycling time of 11-15 min. Interestingly, the presence of transferrin receptor 2 was also associated with uptake of non-transferrin-bound iron which was inhibited by unlabelled transferrin-bound metals. Knockdown reduced transferrin-bound and non-transferrin-bound iron uptake by approximately 60%. Conclusions: Transferrin receptor 2 mediates transferrin-bound iron uptake by receptor-mediated endocytosis. It is also involved in the uptake of non-transferrin-bound iron and the inhibition of non-transferrin-bound iron uptake by diferric transferrin in CHO cells.
Details
- Title
- Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron
- Authors/Creators
- R.M. Graham (Author/Creator) - Fremantle HospitalG.M. Reutens (Author/Creator) - The University of Western AustraliaC.E. Herbison (Author/Creator) - Fremantle HospitalR.D. Delima (Author/Creator) - Fremantle HospitalA.C.G. Chua (Author/Creator) - Fremantle HospitalJ.K. Olynyk (Author/Creator) - Murdoch UniversityD. Trinder (Author/Creator) - Murdoch University
- Publication Details
- Journal of Hepatology, Vol.48(2), pp.327-334
- Publisher
- Elsevier
- Identifiers
- 991005542491807891
- Copyright
- © 2007 European Association for the Study of the Liver
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.184 Physiology & Metals
- 1.184.573 Iron Metabolism
- Web Of Science research areas
- Gastroenterology & Hepatology
- ESI research areas
- Clinical Medicine