Journal article
Transgenic yellow lupin (Lupinus luteus)
Plant Cell Reports, Vol.19(6), pp.634-637
2000
Abstract
Transgenic yellow lupin (Lupinus luteus L.) plants have been generated by meristem co-cultivation with Agrobacterium tumefaciens. The binary plasmid pPZBNIa contains the bar gene under the control of a CaMV 35 S promoter. The transformation method involves inoculation of embryonic axis explants with A. tumefaciens, flooding the meristem with glufosinate, and initial culture on non-selective medium. Shoots were transferred to culture medium containing 20 mg/l glufosinate. Following subculture, shoots were grafted onto non-transgenic narrow-leafed lupin (L. angustifolius L.) seedling rootstocks, or rooted in vitro. The overall transformation efficiency, as determined at the T1 generation, was 0.05%–0.75%. The transgenic nature of plants grown to the T6 generation was confirmed by phosphinothricin acetyl transferase, PCR and Southern analyses.
Details
- Title
- Transgenic yellow lupin (Lupinus luteus)
- Authors/Creators
- H. Li (Author/Creator)S.J. Wylie (Author/Creator)M.G.K. Jones (Author/Creator)
- Publication Details
- Plant Cell Reports, Vol.19(6), pp.634-637
- Publisher
- Springer Verlag
- Identifiers
- 991005541818207891
- Copyright
- © Springer-Verlag Berlin Heidelberg 2000
- Murdoch Affiliation
- State Agricultural Biotechnology Centre; School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.119 Micropropagation
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science