Journal article
Transient gene expression in aleurone protoplasts isolated from developing caryopses of barley and wheat
Plant Molecular Biology, Vol.13(1), pp.21-29
1989
Abstract
Methods have been developed for the isolation of aleurone protoplasts from developing caryopses of Hordeum vulgare and Triticum aestivum in order to study transient expression of introduced genes. Chimaeric gene constructs were introduced into aleurone protoplasts by polyethylene glycol (PEG). Transient expression directed by the 35S promoter from cauliflower mosaic virus (CaMV) of the reporter gene encoding chloramphenicol acetyl transferase (CAT) was detected in aleurone protoplasts from developing barley and wheat grains. Using a similar construct, CAT activity increased when the alcohol dehydrogenase intron 1 fragment from maize was ligated between the 35S promoter and the CAT coding region. The demonstration of transient expression in protoplasts from developing aleurone layers indicates that they may be useful for investigating tissue and developmental control of genes coding for cereal seed proteins.
Details
- Title
- Transient gene expression in aleurone protoplasts isolated from developing caryopses of barley and wheat
- Authors/Creators
- B. Lee (Author/Creator)K. Murdoch (Author/Creator)J. Topping (Author/Creator)M. Kreis (Author/Creator)M.G.K. Jones (Author/Creator)
- Publication Details
- Plant Molecular Biology, Vol.13(1), pp.21-29
- Publisher
- Kluwer Academic Publishers
- Identifiers
- 991005542556307891
- Copyright
- © 1989 Kluwer Academic Publishers
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.119 Micropropagation
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Plant Sciences
- ESI research areas
- Plant & Animal Science