Journal article
Tryptophan and arginine catabolic enzymes and regulatory cytokines in clinically isolated syndrome and multiple sclerosis
Clinical & Translational Immunology, Vol.7(8)
2018
Abstract
Objectives
Clinically isolated syndrome (CIS) is the earliest clinical episode in multiple sclerosis (MS). A study of circulating cells from patients with CIS may help us understand the transition to, and processes associated with, the development of MS.
Methods
As immune cell activity can be determined by flux through metabolic pathways, the mRNA expression of l‐tryptophan‐ and l‐arginine‐catabolising enzymes, indoleamine 2,3‐dioxygenase (IDO) 1 and IDO2 and arginase (ARG) 1 and ARG2, respectively, was compared between peripheral blood mononuclear cells (PBMCs) from healthy controls, and patients with CIS and definite MS. As one measure of cell function, cytokine mRNA levels were analysed directly ex vivo and in cells after culture for 4 h in the absence of regulatory factors in autologous serum.
Results
When measured directly ex vivo, the expression of IDO and ARG was greater in cells from patients with CIS and MS than cells from healthy controls. Although not linked to IDO and ARG expression, PBMCs from the CIS patients were characterised by low IL‐10 and TGFB mRNA levels and not by greater expression of proinflammatory cytokines. When the cells were cultured for 4 h without autologous serum, pro‐ and anti‐inflammatory cytokine mRNA levels positively correlated with IDO1 expression, and TGFB mRNA levels correlated with ARG1 expression.
Conclusion
Higher IDO and ARG expression in CIS and MS provides one sustained homeostatic mechanism to control MS‐associated inflammation. However, potent extrinsic mediators in serum may regulate immune cell function in CIS and associations between IDO, ARG and cytokine expression.
Details
- Title
- Tryptophan and arginine catabolic enzymes and regulatory cytokines in clinically isolated syndrome and multiple sclerosis
- Authors/Creators
- L. Cha (Author/Creator) - The Kids Research Institute AustraliaA.P. Jones (Author/Creator) - The Kids Research Institute AustraliaS. Trend (Author/Creator) - The Kids Research Institute AustraliaS.N. Byrne (Author/Creator) - Westmead Institute for Medical ResearchM.J. Fabis-Pedrini (Author/Creator) - Perron Institute for Neurological and Translational ScienceW.M. Carroll (Author/Creator) - Perron Institute for Neurological and Translational ScienceR.M. Lucas (Author/Creator) - Australian National UniversityJ.M. Cole (Author/Creator) - St John of God Subiaco HospitalD.R. Booth (Author/Creator) - Westmead Institute for Medical ResearchA.G. Kermode (Author/Creator) - Perron Institute for Neurological and Translational ScienceP.H. Hart (Author/Creator) - The Kids Research Institute Australia
- Publication Details
- Clinical & Translational Immunology, Vol.7(8)
- Publisher
- John Wiley & Sons Australia
- Identifiers
- 991005540880107891
- Copyright
- © 2018 The Authors.
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.231 Vitamin Metabolism
- 1.231.1938 Tryptophan Metabolism
- Web Of Science research areas
- Immunology
- ESI research areas
- Immunology