Two Species of Canine Babesia in Australia: Detection and Characterization by PCR

R. Jefferies; U. Ryan; C.J. Muhlnickel; P.J. Irwin

doi:10.1645/0022-3395(2003)089[0409:TSOCBI]2.0.CO;2

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Two Species of Canine Babesia in Australia: Detection and Characterization by PCR

Journal article

Open access

Peer reviewed

Two Species of Canine Babesia in Australia: Detection and Characterization by PCR

R. Jefferies, U. Ryan, C.J. Muhlnickel and P.J. Irwin

Journal of Parasitology, Vol.89(2), pp.409-412

04/2003

DOI: https://doi.org/10.1645/0022-3395(2003)089[0409:TSOCBI]2.0.CO;2

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Abstract

The haemoprotozoan Babesia canis has been recognized in Australia for many years, and a second, smaller species has recently been discovered. Amplification and sequencing of a partial region of the 18S small subunit ribosomal RNA (rRNA) gene enabled detection and characterization of the large and small canine babesiae of Australia for the first time. Isolates from northern Australia were genetically characterized to be 99% homologous to Babesia canis vogeli, confirming previous speculation about the subspecies of B. canis endemic to Australia. The partial 18S rRNA gene sequence amplified from isolates obtained in southeastern Australia was genetically identical to Babesia gibsoni, a species not previously known in Australia. The polymerase chain reaction (PCR) used was shown to be specific to Babesia and had a high sensitivity, detecting DNA at a parasitemia of approximately 0.0000027%. This study also reports the first known detection and characterization of B. canis DNA in Rhipicephalus sanguineus ticks using PCR.

Details

Title: Two Species of Canine Babesia in Australia: Detection and Characterization by PCR
Authors/Creators: R. Jefferies (Author/Creator)
U. Ryan (Author/Creator)
C.J. Muhlnickel (Author/Creator)
P.J. Irwin (Author/Creator)
Publication Details: Journal of Parasitology, Vol.89(2), pp.409-412
Publisher: American Society of Parasitologists
Identifiers: 991005544254907891
Copyright: © 2003 American Society of Parasitologists
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.258 Zoonotic Diseases; 1.258.227 Tick-borne Pathogens
Web Of Science research areas: Parasitology
ESI research areas: Microbiology