Files and links (1)
CC BY V4.0, Open Access
Journal article
Two-Step Concentration of Complex Water Samples for the Detection of Viruses
Methods and protocols, Vol.1(3), 35
2018
PMCID: PMC6481077
PMID: 31164575
Abstract
The accurate detection and quantification of pathogenic viruses in water is essential to understand and reduce the risk of human infection. This paper describes a two-step method suitable for concentrating viruses in water and wastewater samples. The method involves a tangential flow ultrafiltration step that reduces the sample volume of 1–10 L to approximately 50 mL, followed by secondary precipitation using polyethylene glycol 6000, which reduces the volume to 1–4 mL. For method validation, water samples were spiked with different concentrations of enteric viruses, and viral recovery in the concentrates exceeded 10% in all experiments. The method is suitable for water samples with high and low salinity and turbidity, allowing an accurate comparison of viral titers in a diverse range of water types. Furthermore, the method has the potential to concentrate other pathogens, e.g., bacteria or protozoa. Hence, the use of this method can improve the holistic assessment of risks associated with wastewater-contaminated environments.
Details
- Title
- Two-Step Concentration of Complex Water Samples for the Detection of Viruses
- Authors/Creators
- Kata Farkas - Bangor UniversityJames E. McDonaldShelagh K. Malham - Bangor UniversityDavey L. Jones
- Publication Details
- Methods and protocols, Vol.1(3), 35
- Publisher
- MDPI
- Identifiers
- 991005560342607891
- Copyright
- © 2018 by the authors.
- Murdoch Affiliation
- Centre for Sustainable Farming Systems
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Metrics
42 Record Views