Journal article
Tympanic membrane derived stem cell-like cultures for tissue regeneration
Stem Cells and Development, Vol.27(10), pp.649-657
2018
Abstract
Epidermal cells with stem cell-like characteristics have been identified in the tympanic membrane (TM) and localized specifically to the umbo and annulus regions. While they have been proposed to play a role in the regeneration of both acute and chronic TM perforations, evidence for the mechanism and regulation of their contribution is not yet described. Indeed, the behavior of these putative stem cells is largely unknown, in part due to a lack of refined methods for efficient cell isolation. In this study, we compared different explant techniques using normal and perforated rat TM tissues and investigated their ex vivo characteristics. TM after perforation in vivo showed increased staining for epidermal stem cell markers integrin β1 and cytokeratin (CK) 19, and for proliferation Ki-67, indicating activation of the proliferative centers. A mixed population of fibroblasts and epithelial cells were isolated from explant cultures. Excised TM umbo implanted on a culture well insert was the most effective technique. Explants made from perforated TM produced cells before those from unperforated TM. More importantly, the implanted TM umbo organoid was capable of producing cells in a continuous manner, allowing subsequent harvest using trypsin. Primary rat TM epithelial cell cultures positive for pancytokeratin had colony forming activity and could be enriched for CK 19-positive cells that were capable of culture expansion by proliferation and cell migration when subject to a wound assay. Taken together, trauma to the TM activated the proliferative centers and prompted early cell production from TM umbo organoid cultures, which produced TM stem cell-like cultures that proved suitable for tissue engineering of the TM.
Details
- Title
- Tympanic membrane derived stem cell-like cultures for tissue regeneration
- Authors/Creators
- L.J. Liew (Author/Creator) - The University of Western AustraliaL.Q. Chen (Author/Creator) - Murdoch UniversityA.Y. Wang (Author/Creator) - The University of Western AustraliaM. von Unge (Author/Creator) - Akershus University HospitalM.D. Atlas (Author/Creator) - The University of Western AustraliaR.J. Dilley (Author/Creator)
- Publication Details
- Stem Cells and Development, Vol.27(10), pp.649-657
- Publisher
- Mary Ann Liebert Inc.
- Identifiers
- 991005542152807891
- Copyright
- © 2018 Mary Ann Liebert, Inc.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.150 Hearing Loss
- 1.150.1187 Ear Surgery
- Web Of Science research areas
- Cell & Tissue Engineering
- Hematology
- Medicine, Research & Experimental
- Transplantation
- ESI research areas
- Clinical Medicine