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Type I interferon gene therapy protects against cytomegalovirus-induced myocarditis
Journal article   Peer reviewed

Type I interferon gene therapy protects against cytomegalovirus-induced myocarditis

V.S. Cull, E.J. Bartlett and C.M. James
Immunology, Vol.106(3), pp.428-437
2002
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Abstract

Type I interferons (IFNs) are produced early in response to viral infection and modulate adaptive immunity. Previously we demonstrated localized protection against murine cytomegalovirus (MCMV) infection in IFN DNA-inoculated mice. Here we examine the effect of seven IFN subtypes (IFNA1, A2, A4, A5, A6, A9 and B), administered by DNA inoculation, on systemic MCMV infection and myocarditis. IFN transgene expression altered the pathogenesis of MCMV infection with regard to virus titre and myocarditis. IFNA6 treatment reduced MCMV replication whilst IFNA5 and A2 enhanced virus replication. IFNA6, A9, and B treatment inhibited acute myocarditis. A T helper type 1-like, antibody and cytokine, response correlated with decreased virus titre and myocarditis. In addition, IFNA6 was able to reduce chronic cardiac inflammation. This research into the effectiveness of seven type I IFNs, using DNA gene therapy, highlights the need for correct subtype usage in the treatment of disease. We demonstrate effective subtypes for treatment in both the acute and chronic phases of MCMV infection and the resultant development of myocarditis.

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Citation topics
1 Clinical & Life Sciences
1.37 Cardiology - General
1.37.1920 Myocarditis
Web Of Science research areas
Immunology
ESI research areas
Immunology
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