Journal article
Ultra-sensitive class I tetramer analysis reveals previously undetectable populations of antiviral CD8+ T cells
European Journal of Immunology, Vol.34(6), pp.1570-1577
2004
Abstract
A major breakthrough in cellular immunology has been the development of HLA class I tetramers to analyze CD8+ T cell responses. However, in many situations, including persistent virusinfection, specific T cell responses are rarely detected using this technology. This raises the question of whether such responses are ‘deleted’ (or ‘exhausted’) or present below the conventional detection limit for class I tetramer staining. In particular, persistent hepatitis C virus (HCV) infection is characterized by very weak or apparently absent specific CD8+ T cell responses, even though they are readily detectable in acute disease. Therefore, we assessed the use of anti-PE-labeled magnetic beads to enrich tetramer-positive HCV-specific T cells and identify previously undetectable populations. Using the enrichment technique, HCV-specific T cells could be detected in the majority of infected individuals, whereas these responses were not detected using conventional tetramer staining (8/15 vs. 1/15; p=0.01). Magnetic enrichment could reliably detect very rare HCV-specific responses at frequencies of >0.0011% of CD8+ T cells (∼1/million PBMC), and phenotypic analysis of these rare populations was possible. Therefore, this direct ex vivo technique revealed the persistence of very low frequencies of virus-specific CD8+ T cells during chronic virus infection and is readily transferable to the study of other viral, self- or tumor-specific T cells.
Details
- Title
- Ultra-sensitive class I tetramer analysis reveals previously undetectable populations of antiviral CD8+ T cells
- Authors/Creators
- E. Barnes (Author/Creator) - Medawar Building for Pathogen ResearchS.M. Ward (Author/Creator) - Medawar Building for Pathogen ResearchV.O. Kasprowicz (Author/Creator) - Medawar Building for Pathogen ResearchG. Dusheiko (Author/Creator)P. Klenerman (Author/Creator) - Medawar Building for Pathogen ResearchM. Lucas (Author/Creator) - Medawar Building for Pathogen Research
- Publication Details
- European Journal of Immunology, Vol.34(6), pp.1570-1577
- Publisher
- John Wiley & Sons Ltd.
- Identifiers
- 991005544608307891
- Copyright
- © John Wiley & Sons, Inc.
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
43 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.127 T Cell Regulation
- Web Of Science research areas
- Immunology
- ESI research areas
- Immunology