Journal article
Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation
International Journal of Nanomedicine, Vol.6, pp.2167-2174
2011
Abstract
This preliminary study investigated the use of poly (2-hydroxyethyl methacrylate) (pHEMA) nanoparticles for the delivery of the deoxyribonucleic acid (DNA) vaccine pCAG-HAk, which expresses the full length hemagglutinin (HA) gene of the avian influenza A/Eurasian coot/Western Australian/2727/1979 (H6N2) virus with a Kozak sequence which is in the form of a pCAGGS vector. The loaded and unloaded nanoparticles were characterized using field-emission scanning electron microscopy. Further characterizations of the nanoparticles were made using atomic force microscopy and dynamic light scattering, which was used to investigate particle size distributions. This preliminary study suggests that using 100 µg of pHEMA nanoparticles as a nanocarrier/adjuvant produced a reduction in virus shedding and improved the immune response to the DNA vaccine pCAG-HAk.
Details
- Title
- Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation
- Authors/Creators
- G.E.J. Poinern (Author/Creator)X.T. Le (Author/Creator)S. Shan (Author/Creator)T. Ellis (Author/Creator)S. Fenwick (Author/Creator)J. Edwards (Author/Creator)D. Fawcett (Author/Creator)
- Publication Details
- International Journal of Nanomedicine, Vol.6, pp.2167-2174
- Publisher
- Dove Press
- Identifiers
- 991005540790307891
- Copyright
- © 2011 Poinern et al
- Murdoch Affiliation
- School of Engineering and Energy; School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.358 Dendritic Cell Therapy
- Web Of Science research areas
- Nanoscience & Nanotechnology
- Pharmacology & Pharmacy
- ESI research areas
- Pharmacology & Toxicology