Ultrastructure of the epithelial cells associated with tooth biomineralization in the chiton Acanthopleura hirtosa

J.A. Shaw; D.J. Macey; L.R. Brooker; E.J. Stockdale; M. Saunders; P.L. Clode

doi:10.1017/S1431927609090230

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Ultrastructure of the epithelial cells associated with tooth biomineralization in the chiton Acanthopleura hirtosa

Journal article

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Peer reviewed

Ultrastructure of the epithelial cells associated with tooth biomineralization in the chiton Acanthopleura hirtosa

J.A. Shaw, D.J. Macey, L.R. Brooker, E.J. Stockdale, M. Saunders and P.L. Clode

Microscopy and Microanalysis, Vol.15(02), pp.154-165

2009

DOI: https://doi.org/10.1017/S1431927609090230

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Abstract

The cusp epithelium is a specialized branch of the superior epithelium that surrounds the developing teeth of chitons and is responsible for delivering the elements required for the formation of biominerals within the major lateral teeth. These biominerals are deposited within specific regions of the tooth in sequence, making it possible to conduct a row by row examination of cell development in the cusp epithelium as the teeth progress from the unmineralized to the mineralized state. Cusp epithelium from the chiton Acanthopleura hirtosa was prepared using conventional chemical and microwave assisted tissue processing, for observation by light microscopy, conventional transmission electron microscopy (TEM) and energy filtered TEM. The onset of iron mineralization within the teeth, initiated at row 13, is associated with a number of dramatic changes in the ultrastructure of the apical cusp cell epithelium. Specifically, the presence of ferritin containing siderosomes, the position and number of mitochondria, and the structure of the cell microvilli are each linked to aspects of the mineralization process. These changes in tissue development are discussed in context with their influence over the physiological conditions within both the cells and extracellular compartment of the tooth at the onset of iron mineralization.

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Title: Ultrastructure of the epithelial cells associated with tooth biomineralization in the chiton Acanthopleura hirtosa
Authors/Creators: J.A. Shaw (Author/Creator) - The University of Western Australia
D.J. Macey (Author/Creator) - Murdoch University
L.R. Brooker (Author/Creator) - University of the Sunshine Coast
E.J. Stockdale (Author/Creator) - Centre for Microscopy, Characterisation and Analysis
M. Saunders (Author/Creator) - Centre for Microscopy, Characterisation and Analysis
P.L. Clode (Author/Creator) - Centre for Microscopy, Characterisation and Analysis
Publication Details: Microscopy and Microanalysis, Vol.15(02), pp.154-165
Publisher: Cambridge University Press
Identifiers: 991005543717107891
Copyright: (c) Microscopy Society of America
Murdoch Affiliation: School of Biological Sciences and Biotechnology
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 2 Chemistry; 2.165 Nanofibers, Scaffolds & Fabrication; 2.165.1082 Calcium Carbonate Crystallization
Web Of Science research areas: Materials Science, Multidisciplinary; Microscopy
ESI research areas: Materials Science