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Journal article
Underrepresentation of activating KIR gene expression in single‐cell RNA‐seq data is due to KIR gene misassignment
European journal of immunology, e2350590
2023
PMID: 37944995
Abstract
Standard single-cell RNA-sequencing alignment pipelines exhibit a propensity for misassigning killer immunoglobulin-like receptor (KIR) transcripts, thereby giving rise to inaccuracies in quantifying KIR expression. Alves et al. elucidated that these default workflows frequently misclassify activating KIR transcripts as inhibitory KIR expression, resulting in a skewed representation of the KIR repertoire.
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Details
- Title
- Underrepresentation of activating KIR gene expression in single‐cell RNA‐seq data is due to KIR gene misassignment
- Authors/Creators
- Eric Alves - The University of Western AustraliaAbha Chopra - Murdoch UniversityRamesh Ram - Institute for Immunology and Infectious Diseases Murdoch University Murdoch Western Australia AustraliaJennifer Currenti - Harry Perkins Institute of Medical ResearchSpyros A. Kalams - Vanderbilt University Medical CenterSimon A. Mallal - Murdoch UniversityElizabeth J. Phillips - Murdoch UniversitySilvana Gaudieri - Murdoch University
- Publication Details
- European journal of immunology, e2350590
- Publisher
- John Wiley & Sons, Inc.
- Identifiers
- 991005614807807891
- Copyright
- © 2023 The Authors.
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases; Centre for Molecular Medicine and Innovative Therapeutics
- Language
- English
- Resource Type
- Journal article
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