Journal article
Understanding the molecular epidemiology of the footrot pathogen Dichelobacter nodosus to support control and eradication programs
Journal of Clinical Microbiology, Vol.48(3), pp.877-882
2010
Abstract
The Gram-negative anaerobe Dichelobacter nodosus is the primary etiologic agent of ovine footrot. Few studies of the genetic diversity and epidemiology of D. nodosus have been done, despite the economic cost and welfare implications of the disease. This study examined a large collection of Australian isolates; 735 isolates from footrot-infected sheep from 247 farms in Western Australia (WA) were tested by pulsed-field gel electrophoresis (PFGE), and a subset of 616 isolates was tested by infrequent restriction site PCR (IRS-PCR). The genetic diversity of WA isolates was compared to that of 61 isolates from three other Australian states. WA isolates were genetically diverse, with 181 molecular types resolved by PFGE, resulting in a simple diversity ratio (SDR) of 1:4 and a Simpson's index of discrimination value (D) of 0.98. IRS-PCR resolved 77 molecular types (SDR = 1:8 and D = 0.95). The isolates were grouped into 67 clonal groups by PFGE (SDR = 1:11, D = 0.90) and 36 clonal groups by IRS-PCR (SDR = 1:17, D = 0.87). Despite the high genetic diversity, three common clonal groups predominated in WA and were found in other Australian states. On some farms, molecular type was stable over a number of years, whereas on other farms genetically diverse isolates occurred within a flock of sheep or within a hoof. This study provides a large database from which to appropriately interpret molecular types found in epidemiological investigations and to identify common and unknown types that may compromise footrot eradication or control programs.
Details
- Title
- Understanding the molecular epidemiology of the footrot pathogen Dichelobacter nodosus to support control and eradication programs
- Authors/Creators
- N. B. Buller (Author/Creator) - Department of Agriculture Western Australia, South Perth, Western Australia 6151P. Ashley (Author/Creator) - Department of Agriculture Western Australia, South Perth, Western Australia 6151M. Palmer (Author/Creator) - Australian Reference Laboratory for Ovine Footrot, Department of Agriculture Western Australia, Albany, Western Australia 6330D. Pitman (Author/Creator) - Australian Reference Laboratory for Ovine Footrot, Department of Agriculture Western Australia, Albany, Western Australia 6330R. B. Richards (Author/Creator) - Australian Reference Laboratory for Ovine Footrot, Department of Agriculture Western Australia, Albany, Western Australia 6330D.J. Hampson (Author/Creator) - Murdoch University
- Publication Details
- Journal of Clinical Microbiology, Vol.48(3), pp.877-882
- Publisher
- American Society for Microbiology
- Identifiers
- 991005540508307891
- Copyright
- © 2010, American Society for Microbiology
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences; Harry Butler Institute
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.51 Dairy & Animal Sciences
- 3.51.799 Farm Animal Welfare
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology