Journal article
Upregulation of 15 antisense long non-coding RNAs in osteosarcoma
Genes, Vol.12(8), Art. 1132
2021
Abstract
The human genome encodes thousands of natural antisense long noncoding RNAs (lncRNAs); they play the essential role in regulation of gene expression at multiple levels, including replication, transcription and translation. Dysregulation of antisense lncRNAs plays indispensable roles in numerous biological progress, such as tumour progression, metastasis and resistance to therapeutic agents. To date, there have been several studies analysing antisense lncRNAs expression profiles in cancer, but not enough to highlight the complexity of the disease. In this study, we investigated the expression patterns of antisense lncRNAs from osteosarcoma and healthy bone samples (24 tumour-16 bone samples) using RNA sequencing. We identified 15 antisense lncRNAs (RUSC1-AS1, TBX2-AS1, PTOV1-AS1, UBE2D3-AS1, ERCC8-AS1, ZMIZ1-AS1, RNF144A-AS1, RDH10-AS1, TRG-AS1, GSN-AS1, HMGA2-AS1, ZNF528-AS1, OTUD6B-AS1, COX10-AS1 and SLC16A1-AS1) that were upregulated in tumour samples compared to bone sample controls. Further, we performed real-time polymerase chain reaction (RT-qPCR) to validate the expressions of the antisense lncRNAs in 8 different osteosarcoma cell lines (SaOS-2, G-292, HOS, U2-OS, 143B, SJSA-1, MG-63, and MNNG/HOS) compared to hFOB (human osteoblast cell line). These differentially expressed IncRNAs can be considered biomarkers and potential therapeutic targets for osteosarcoma
Details
- Title
- Upregulation of 15 antisense long non-coding RNAs in osteosarcoma
- Authors/Creators
- E. Rothzerg (Author/Creator) - The University of Western AustraliaX.D. Ho (Author/Creator) - Hue UniversityJ. Xu (Author/Creator) - The University of Western AustraliaD. Wood (Author/Creator) - The University of Western AustraliaA. Märtson (Author/Creator) - Tartu University HospitalS. Kõks (Author/Creator) - Perron Institute for Neurological and Translational Science
- Publication Details
- Genes, Vol.12(8), Art. 1132
- Publisher
- MDPI
- Identifiers
- 991005543879407891
- Copyright
- © 2021 The Authors.
- Murdoch Affiliation
- Centre for Molecular Medicine and Innovative Therapeutics
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
12 File views/ downloads
45 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.196 Micro & Long Noncoding RNA
- 1.196.921 lncRNA
- Web Of Science research areas
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics