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Uptake and oxidation of aromatic substrates by Rhizobium leguminosarum MNF 3841 and Rhizobium trifolii TA1
Journal article   Peer reviewed

Uptake and oxidation of aromatic substrates by Rhizobium leguminosarum MNF 3841 and Rhizobium trifolii TA1

Y.P. Chen, A.R. Glenn and M.J. Dilworth
FEMS Microbiology Letters, Vol.21(2), pp.201-205
1984
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Abstract

Rhizobium trifolii TA1 and Rhizobium leguminosarum MNF 3841 grow on a range of aromatic substrates. R. trifolii TA1 possesses enzymes of both the catechol and protocatechuate pathways, whereas R. leguminosarum MNF 3841 only has enzymes of the latter pathway. The pathways are induced by growth on benzoate or 4-hydroxybenzoate, respectively, but they are not cross-inducible. 4-Hydroxybenzoate permease and hydroxylase are induced by growth on 4-hydroxybenzoate but not on protocatechuate, suggesting that they are regulated separately from protocatechuate dioxygenase. The uptake systems for both benzoate and 4-hydroxybenzoate are inhibited by azide, carbonyl cyanide m-chlorophenyl hydrazone and N, N′-dicyclohexylcarbodiimide but are insensitive to arsenate. Salicylate and protocatechuate interfere with benzoate and 4-hydroxybenzoate uptake, respectively.

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