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Journal article
Urinary Metabolic Profiling of Liver Fluke-Induced Cholangiocarcinoma-A Follow-Up Study
Journal of clinical and experimental hepatology, Vol.13(2), pp.203-217
2023
PMID: 36950498
Abstract
Background/Aims: Global liquid chromatography mass spectrometry (LC-MS) profiling in a Thai population has previously identified a urinary metabolic signature in Opisthorchis viverrini-induced cholangiocarcinoma (CCA), primarily characterised by disturbance in acylcarnitine, bile acid, steroid, and purine metabolism. However, the detection of thousands of analytes by LC-MS in a biological sample in a single experiment potentially introduces false discovery errors. To verify these observed metabolic perturbations, a second validation dataset from the same population was profiled in a similar fashion. Methods: Reverse-phase ultra-performance liquid -chromatog-raphy mass spectrometry was utilised to acquire the global spectral profile of 98 spot urine samples (from 46 healthy volunteers and 52 CCA patients) recruited from Khon Kaen, northeast Thailand (the highest incidence of CCA globally). Results: Metabolites were differentially expressed in the urinary profiles from CCA patients. High urinary elimination of bile acids was affected by the presence of obstructive jaundice. The urine metabo-lome associated with non-jaundiced CCA patients showed a distinctive pattern, similar but not identical to pub-lished studies. A panel of 10 metabolites achieved a diagnostic accuracy of 93.4% and area under the curve value of 98.8% (CI = 96.3%-100%) for the presence of CCA. Conclusions: Global characterisation of the CCA urinary metab-olome identified several metabolites of biological interest in this validation study. Analyses of the diagnostic util-ity of the discriminant metabolites showed excellent diagnostic potential. Further larger scale studies are required to confirm these findings internationally, particularly in comparison to sporadic CCA, not associated with liver fluke infestation.
Details
- Title
- Urinary Metabolic Profiling of Liver Fluke-Induced Cholangiocarcinoma-A Follow-Up Study
- Authors/Creators
- Munirah Alsaleh - St Mary's HospitalPaiboon Sithithaworn - Khon Kaen UniversityNarong Khuntikeo - Khon Kaen UniversityWatcharin Loilome - Khon Kaen UniversityPuangrat Yongvanit - Khon Kaen UniversityThomas Hughes - St Mary's HospitalThomas O'Connor - St Mary's HospitalRoss H. Andrews - Khon Kaen UniversityChristopher A. Wadsworth - St Mary's HospitalRoger Williams - King's College LondonLarry Koomson - St Mary's HospitalIsobel Jane Cox - King's College LondonElaine Holmes - St Mary's HospitalSimon D. Taylor-Robinson - St Mary's Hospital
- Publication Details
- Journal of clinical and experimental hepatology, Vol.13(2), pp.203-217
- Publisher
- Elsevier
- Number of pages
- 15
- Grant note
- AMMF - the Cholangiocarcinoma Charity (Stansted, Essex, UK) StratiGrad PhD program at Imperial College London Wellcome Trust ISSF Fund at Imperial College London; Wellcome Trust
- Identifiers
- 991005884953107891
- Copyright
- © 2022 Indian National Association for Study of the Liver.
- Murdoch Affiliation
- Centre for Computational and Systems Medicine
- Language
- English
- Resource Type
- Journal article
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