Journal article
Urinary neutrophil gelatinase-associated lipocalin concentration changes after acute haemorrhage and colloid-mediated reperfusion in anaesthetized dogs
Veterinary Anaesthesia and Analgesia, Vol.43(3), pp.262-270
2016
Abstract
Objective: To determine changes in urine neutrophil gelatinase-associated lipocalin concentration (uNGAL) in anaesthetized Greyhound dogs that developed acute tubular damage following haemorrhage and resuscitation with colloid-based fluids. Study design: Prospective experimental study. Animals: Seven healthy adult entire male Greyhound dogs. Methods: During isoflurane anaesthesia, approximately 50 mL kg-1 of blood was removed to maintain mean arterial pressure (MAP) ≤40 mmHg for 1 hour followed by gelatin-based colloid administration to maintain MAP ≥60 mmHg for 3 hours. Data, including oxygen extraction ratio and uNGAL, were collected before (T0) and immediately following (T1) haemorrhage, and hourly during reperfusion (T2-T4). After T4, dogs were euthanized and renal tissue was collected for histology. Statistical analysis was performed using repeated-measures one-way anova. Data are presented as means (95% confidence interval). Results: Histology identified renal tubular epithelial damage in all dogs. Urine NGAL concentration increased from 12.1 (0-30.6) ng mL-1 at T0 to 122.0 (64.1-180.0) ng mL-1 by T3. Compared with T0, uNGAL was significantly higher at T3 (p = 0.016) and was increased 24-fold. Conclusions and clinical relevance: Despite wide individual variation in baseline uNGAL, increases in uNGAL were observed in all dogs, suggesting that this biomarker has the potential to detect renal tubular injury following haemorrhage-induced hypotension and colloid-mediated reperfusion.
Details
- Title
- Urinary neutrophil gelatinase-associated lipocalin concentration changes after acute haemorrhage and colloid-mediated reperfusion in anaesthetized dogs
- Authors/Creators
- J. Davis (Author/Creator)A.L. Raisis (Author/Creator)R.E. Cianciolo (Author/Creator)D.W. Miller (Author/Creator)R.E. Shiel (Author/Creator)M.B. Nabity (Author/Creator)G.L. Hosgood (Author/Creator)
- Publication Details
- Veterinary Anaesthesia and Analgesia, Vol.43(3), pp.262-270
- Publisher
- Blackwell Publishing Inc.
- Identifiers
- 991005544830407891
- Copyright
- © 2015 Association of Veterinary Anaesthetists and the American College of Veterinary Anesthesia and Analgesia
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
80 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.55 Urology & Nephrology - General
- 1.55.830 Acute Kidney Injury
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science