Journal article
Use of Autostitch for automatic stitching of microscope images
Micron (1969), Vol.38(5), pp.492-499
2007
Abstract
Image stitching is the process of combining multiple images to produce a panorama or larger image. In many biomedical studies, including those of cancer and infection, the use of this approach is highly desirable in order to acquire large areas of certain structures or whole sections, while retaining microscopic resolution. In this study, we describe the application of Autostitch, viz. software that is normally used for the generation of panoramas in photography, in the seamless stitching of microscope images. First, we tested this software on image sets manually acquired by normal light microscopy and compared the performance with a manual stitching approach performed with Paint Shop Pro. Secondly, this software was applied to an image stack acquired by an automatic microscope. The stitching results were then compared with that generated by a self-programmed rectangular tiling macro integrated in Image J. Thirdly, this program was applied in the image stitching of images from electron microscopy. Thus, the automatic stitching program described here may find applications in convenient image stitching and virtual microscopy in the biomedical research.
Details
- Title
- Use of Autostitch for automatic stitching of microscope images
- Authors/Creators
- B. Ma (Author/Creator)T. Zimmermann (Author/Creator)M. Rohde (Author/Creator)S. Winkelbach (Author/Creator)F. He (Author/Creator)W. Lindenmaier (Author/Creator)K.E.J. Dittmar (Author/Creator)
- Publication Details
- Micron (1969), Vol.38(5), pp.492-499
- Publisher
- Elsevier Ltd.
- Identifiers
- 991005545098007891
- Copyright
- © 2006 Elsevier Ltd.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
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- Citation topics
- 4 Electrical Engineering, Electronics & Computer Science
- 4.116 Robotics
- 4.116.133 Simultaneous Localization and Mapping
- Web Of Science research areas
- Microscopy
- ESI research areas
- Biology & Biochemistry