Use of DNA amplification for the rapid identification of Mycobacterium bovis

D.V. Cousins; S.D. Wilton; B.R. Francis

doi:10.1016/0378-1135(91)90010-D

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Use of DNA amplification for the rapid identification of Mycobacterium bovis

Journal article

Peer reviewed

Use of DNA amplification for the rapid identification of Mycobacterium bovis

D.V. Cousins, S.D. Wilton and B.R. Francis

Veterinary Microbiology, Vol.27(2), pp.187-195

1991

DOI: https://doi.org/10.1016/0378-1135(91)90010-D

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Abstract

DNA amplification using the polymerase chain reaction technique was evaluated for rapid identification of Mycobacterium bovis. Two oligonucleotide primers of 20 bases in length were constructed to target a region of the gene encoding the M. bovis secretory protein, MPB70. The amplification reaction produced a single product 372 bp in size which was readily detected by agarose gel electrophoresis. All 84 strains of M. bovis tested produced a positive signal in the amplification reaction. In addition all isolates fro the M. tuberculosis complex tested, with the exception of M. microti, gave a single band at 372 bp. No amplified product was detected when 24 other species of mycobacteria and species from four other genera were tested. The sensitivity of the test was such that a single viable cell could be detected in the reaction. This technique provides a simple and extremely sensitive method of identifying isolates of M. bovis and other pathogenic M. tuberculosis complex organisms.

Details

Title: Use of DNA amplification for the rapid identification of Mycobacterium bovis
Authors/Creators: D.V. Cousins (Author/Creator) - Animal Health Laboratories, Department of Agriculture, South Perth, Australia.
S.D. Wilton (Author/Creator) - Biotech Int. Ltd., Brodie Hall Drive, Bentley, W.A. 6102, Australia
B.R. Francis (Author/Creator) - Government of Western Australia
Publication Details: Veterinary Microbiology, Vol.27(2), pp.187-195
Publisher: Elsevier BV
Identifiers: 991005540020007891
Copyright: 1991 Elsevier BV
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.194 Tuberculosis & Leprosy; 1.194.273 TB Diagnostics & Treatment
Web Of Science research areas: Microbiology; Veterinary Sciences
ESI research areas: Plant & Animal Science