Use of RAPD-PCR to isolate a species specific DNA probe forPhytophthora cinnamomi

M.P. Dobrowolski; P.A. O'Brien

doi:10.1111/j.1574-6968.1993.tb06485.x

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Use of RAPD-PCR to isolate a species specific DNA probe forPhytophthora cinnamomi

Journal article

Peer reviewed

Use of RAPD-PCR to isolate a species specific DNA probe forPhytophthora cinnamomi

M.P. Dobrowolski and P.A. O'Brien

FEMS Microbiology Letters, Vol.113(1), pp.43-47

1993

DOI: https://doi.org/10.1111/j.1574-6968.1993.tb06485.x

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Abstract

The products of RAPD-PCR amplification of Phytophthora cinnamomi DNA were separated by electrophoresis in agarose. Parallel Southern blots of the gels were hybridized with nick translated DNA from different species of Phytophthora. Fragments that hybridized specifically to P. cinnamomi DNA were identified. These fragments were purified and cloned into pUC18. Their specificity for P. cinnamomi was confirmed.

Details

Title: Use of RAPD-PCR to isolate a species specific DNA probe forPhytophthora cinnamomi
Authors/Creators: M.P. Dobrowolski (Author/Creator)
P.A. O'Brien (Author/Creator)
Publication Details: FEMS Microbiology Letters, Vol.113(1), pp.43-47
Publisher: Blackwell Publishing
Identifiers: 991005542670907891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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