Use of RAPD-PCR to isolate a species specific DNA probe for Phytophthora cinnamomi

M.P. Dobrowolski; P.A. O'Brien

doi:10.1016/0378-1097(93)90096-K

Back

Use of RAPD-PCR to isolate a species specific DNA probe for Phytophthora cinnamomi

Journal article

Peer reviewed

Use of RAPD-PCR to isolate a species specific DNA probe for Phytophthora cinnamomi

M.P. Dobrowolski and P.A. O'Brien

FEMS Microbiology Letters, Vol.113(1), pp.43-47

1993

DOI: https://doi.org/10.1016/0378-1097(93)90096-K

Files and links (1)

url

Link to Published Version *Subscription may be requiredView

Abstract

The products of RAPD-PCR amplification of Phytophthora cinnamomi DNA were separated by electrophoresis in agarose. Parallel Southern blots of the gels were hybridized with nick translated DNA from different species of Phytophthora. Fragments that hybridized specifically to P. cinnamomi DNA were identified. These fragments were purified and cloned into pUC18. Their specificity for P. cinnamomi was confirmed.

Details

Title: Use of RAPD-PCR to isolate a species specific DNA probe for Phytophthora cinnamomi
Authors/Creators: M.P. Dobrowolski (Author/Creator)
P.A. O'Brien (Author/Creator)
Publication Details: FEMS Microbiology Letters, Vol.113(1), pp.43-47
Publisher: Blackwell Publishing
Identifiers: 991005542973407891
Murdoch Affiliation: School of Biological and Environmental Sciences
Language: English
Resource Type: Journal article

Metrics

52 Record Views