Use of a Murine Cytomegalovirus K181-Derived bacterial artificial chromosome as a vaccine vector for immunocontraception

A.J. Redwood; M. Messerle; N.L. Harvey; C.M. Hardy; U.H. Koszinowski; M.A. Lawson; G.R. Shellam

doi:10.1128/JVI.79.5.2998-3008.2005

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Use of a Murine Cytomegalovirus K181-Derived bacterial artificial chromosome as a vaccine vector for immunocontraception

Journal article

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Peer reviewed

Use of a Murine Cytomegalovirus K181-Derived bacterial artificial chromosome as a vaccine vector for immunocontraception

A.J. Redwood, M. Messerle, N.L. Harvey, C.M. Hardy, U.H. Koszinowski, M.A. Lawson and G.R. Shellam

Journal of Virology, Vol.79(5), pp.2998-3008

2005

DOI: https://doi.org/10.1128/JVI.79.5.2998-3008.2005

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Abstract

Cytomegaloviruses (CMVs) are members of the Betaherpesvirinae subfamily of the Herpesviridae, and their properties of latency, large DNA size, gene redundancy, and ability to be cloned as bacterial artificial chromosomes (BACs) suggest their utility as vaccine vectors. While the K181 strain of murine CMV (MCMV) is widely used to study MCMV biology, a BAC clone of this virus had not previously been produced. We report here the construction of a BAC clone of the K181Perth strain of MCMV. The in vivo and in vitro growth characteristics of virus derived from the K181 BAC were similar to those of wild-type K181. The utility of the K181 BAC as a method for the rapid production of vaccine vectors was assessed. A vaccine strain of BAC virus, expressing the self-fertility antigen, murine zona pellucida 3, was produced rapidly using standard bacterial genetics techniques and rendered female BALB/c mice infertile with a single intraperitoneal inoculation. In addition, attenuated vaccine strains lacking the open reading frames m07 to m12 exhibited no reduction in efficacy compared to the full-length vaccine strain. In conclusion, we describe the production of a K181-based BAC virus which behaved essentially as wild-type K181 and allowed the rapid production of effective viral vaccine vectors.

Details

Title: Use of a Murine Cytomegalovirus K181-Derived bacterial artificial chromosome as a vaccine vector for immunocontraception
Authors/Creators: A.J. Redwood (Author/Creator) - The University of Western Australia
M. Messerle (Author/Creator) - Department of Virology
N.L. Harvey (Author/Creator) - School of Molecular Sciences
C.M. Hardy (Author/Creator) - Cooperative Research Centre
U.H. Koszinowski (Author/Creator) - Department of Virology
M.A. Lawson (Author/Creator) - Animal Care Services
G.R. Shellam (Author/Creator) - School of Pathology and Laboratory Medicine
Publication Details: Journal of Virology, Vol.79(5), pp.2998-3008
Publisher: American Society for Microbiology
Identifiers: 991005541034307891
Copyright: © 2005, American Society for Microbiology.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.161 Virology - Identification & Sequencing; 1.161.711 Cytomegalovirus Infections
Web Of Science research areas: Virology
ESI research areas: Microbiology